Applications of monoclonal antibodies and single chain variable fragments in pharmacognosy
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Abstract
Monoclonal antibody (MAb) is a monospecifcimmunoglobulinproduced by hybridomas. Single chain variable fragment (scFv), a MAb derivative,is a fusion protein of the variable regions of the heavy (VH) and light chains (VL) of immunoglobulins, connected with a short linker peptide.MAbs and scFvs against naturally occurring pharmacological active compounds were prepared and applied to pharmacognosical works, including quality control of medicinal plants by ELISA and Eastern blotting, immunohistochemistry and immunocytochemistry localization of bioactive compounds in plants and cell lines, one-step purifcation by immunoaffnity chromatography, enhancement of medicinal constituents by the new technique named “missile type molecular breeding”.
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References
Afshar S, Asai T, Morrison SL.Humanized ADEPT comprised of an engineered human purine nucleoside phosphorylase and a tumor targeting peptide for treatment of cancer. Mol Cancer Ther 2009; 8(1): 185-93.
Fukuda N, Tanaka H, Shoyama Y. Application of ELISA, Western blotting and immunoaffinity concentration for survey of ginsenosides in crude drugs of Panax species and traditional Chinese herbal medicines. Analyst 2000a; 125: 1425-1429.
Fukuda N, Tanaka H, Shoyama Y. Double staining of ginsenosides by Western blotting using anti-ginsenoside Rb1 and Rg1 monoclonal antibodies. Biol Pharm Bull. 2001; 24(10):1157-60.
Fukuda N, Tanaka H, Shoyama Y. Formation of monoclonal antibody against a major ginseng component, ginsenoside Rg1 and its characterization. Cytotechnology 2000b; 34(3):197-204.
Fukuda N, Tanaka H, Shoyama Y. Isolation of the pharmacologically active saponinginsenosideRb1 from ginseng by immunoaffinity column chromatography. J Nat Prod 2000c; 63(2):283-5.
Holliger P, Hudson PJ.Engineered antibody fragments and the rise of single domains. Nat Biotechnol 2005; 23(9): 1126-1136.
Imsungnoen N, Phrompittayarat W, Ingkaninan K,Tanaka H, Putalun W. Immunochromatographicassay for the detection of pseudojujubogenin glycosides. Phytochem Anal 2009; 20(1):64-67.
Joo EJ, Ha YW, Shin H, Son SH, Kim YS. Generation and Characterization of Monoclonal Antibody to Ginsenoside Rg3. Biol Pharm Bull 2009; 32(4):548—552.
Juengwatanatrakul T, Morinaga O, Tanaka H, Shoyama Y. Production of single chain Fv fragment against sennoside A. Proceeding of the 51thAnnual Meeting of the Japanese Society of Pharmacognosy.9th September 2004, p203.
Juengwatanatrakul T, Sritularak B, Amornnopparat-tanakul P, et al. Preparation of a specific monoclonal antibody to asiaticoside for the development of an enzyme-linked immunosorbent assay. Analyst 2011; 136(5): 1013-1017.
Kido K, Edakuni K, Morinaga O, Tanaka H, Shoyama Y. An enzyme-linked immunosorbent assay for aconitine-type alkaloids using an anti-aconitine monoclonal antibody. Anal Chim Acta 2008a; 616(1):109-114.
Kido K, Morinaga O, Shoyama Y, Tanaka H. Quick analysis of baicalin in Scutellariae Radix by enzyme-linked immunosorbent assay using a monoclonal antibody. Talanta 2008b; 77(1): 346-350.
Kim J, Tanaka H, Shoyama Y. Immunoquantitativeanalysis for berberine and its related com-pounds using monoclonal antibodies in herbal medicines. Analyst 2004a; 129(1): 87-91.
Kim J, Tanaka H, Yuan C, Shoyama Y. Development of monoclonal antibody against isoquinoline alkaloid coptisine and its application for the screening of medicinal plants. Cytotechnology 2004b; 44(3):115-123.
Kobatake E, Yamano R, Mie M. Targeted delivery using immunoliposomes with a lipid-modified antibody-binding protein. Appl BiochemBiotechnol 2011; 163(2):296-303.
Linke S, Neubauer K, Dorner MB, Dorner BG, Pauli G, Schweiger B. Generation and characterisation of monoclonal antibodies against influenza virus A, subtype H5N1. J Virol Methods 2011; 175(1): 85-94.
Loungratana P, Tanaka H, Shoyama Y. Production of monoclonal antibody against ginkgolic acids in Ginkgo biloba Linn. Am J Chin Med 2004; 32(1): 33-48.
Lu Z, Masaki T, Shoyama Y, Tanaka H. Construction and expression of a single chain Fv fragment against pharmacologically active paeoniflorin in Escherichia coli, and its potential use in an enzyme-linked immunosorbent assay. Planta Med 2006; 72(2): 151-155.
Lu Z, Morinaga O, Tanaka H, Shoyama Y. A quantitative ELISA using monoclonal anti-body to survey paeoniflorin and albiflorin in crude drugs and traditional Chinese herbal medicines. Biol Pharm Bull 2003; 26(6):862-866.
Manjappa AS, Chaudhari KR, Venkataraju MP, et al. Antibody derivatization and conjugationstrategies: application in preparation of stealth immunoliposome to target chemotherapeutics to tumor. J Control Release 2011; 150(1):2-22.
Marks JD, Hoogenboom HR, Bonnert TP, Mc CaffertyJ, Griffiths AD, Winter G. Bypassing immunization; Human antibodies from V-gene libraries displayed on phage. J MolBiol 1991; 222:581-597.
Mattos-Arruda LD, Dienstmann R, Tabernero J. Development of Molecular Biomarkers in Individualized Treatment of Colorectal Cancer. ClinColorectal Cancer 2011; [In press]
Morinaga O, Fujino A, Tanaka H, Shoyama Y. Anon-membrane quantitative analysis system for glycyrrhizin in licorice roots and traditional Chinese medicines. Anal BioanalChem 2005a; 383(4):668-672.
Morinaga O, Fukuda N, Tanaka H, Shoyama Y. Chromatographic resolution of glucosidiccompounds, ginsenosides on polyethersulphone membrane, and its application to the quantitative immunoassay for ginseng saponins. Glycobiology 2005; 15(10):1061-1066.
Morinaga O, Nakajima S, Tanaka H, Shoyama Y. Production of monoclonal antibodies against a major purgative component, sennoside A, their characterization and use in ELISA. Analyst 2000; 125: 1109–13.
Morinaga O, Shoyama Y. Development of new staining technology “Eastern blotting” using monoclonal antibody. Current Drug Discovery Technolo-gies 2011; 8:42-50.
Morinaga O, Tanaka H, Shoyama Y. Detection and quantification of ginsenoside Re in ginseng samples by a chromatographic immunostainingmethod using monoclonal antibody against ginsenoside Re. J Chromatogr B AnalytTechnol Biomed Life Sci 2006a; 830(1):100-104.
Morinaga O, Uto T, Sakamoto S, et al. Development of eastern blotting technique for sennoside A and sennoside B using anti-sennoside A and anti-sennoside B monoclonal antibodies. Phytochem Anal 2009a; 20(2):154-8.
Morinaga O, Uto T, Sakamoto S, Tanaka H, Shoyama Y. Enzyme-linked immunosorbent assay for total sennosides using anti-sennside A and anti-sennoside B monoclonal antibodies. Fitoterapia 2009b; 80(1): 28-31.
Morinaga O, Uto T, Yuan CS, Tanaka H, Shoyama Y. Evaluation of a new eastern blotting technique for the analysis of ginsenoside Re in American ginseng berry pulp extracts. Fitoterapia 2010; 81(4): 284-288.
Morinaga O, Zhu S, Tanaka H, Shoyama Y. Visual detection of saikosaponins by on-membrane immunoassay and estimation of traditional Chinese medicines containing Bupleuri radix. Biochem Biophys Res Commun 2006b; 346(3): 687-692.
Morinaga O, Nakajima S, Tanaka H, Shoyama Y. Pro-duction of monoclonal antibodies against a major purgative component, sennoside B, their characterization and use in ELISA, Analyst 2001; 126(8): 1372-6.
Nah JJ, Song JY, Choi S, et al. Preparation of monoclonal antibody against ginsenosideRf and its enzyme immunoassay. Biol Pharm Bull 2000; 23(5):523-6.
Nelson AL. Antibody fragments; Hope and hype.mAbs 2010; 2:77-83.
Paudel MK, Putalun W, Sritularak B, Morinaga O, Shoyama Y, Tanaka H, Morimoto S. Development of a combined technique using a rapid one-step immunochromatographic assay and indirect competitive ELISA for the rapid detection of baicalin. Anal ChimActa 2011;701(2):189-93.
Petráková K. Avastin in the treatment of breast cancer. KlinOnkol 2011; 24(2):101-5.
Phrompittayarat W, Putalun W, Tanaka H, Jetiyanon K,Wittaya-Areekul S, Ingkaninan K. Determination of pseudojujubogenin glycosides from Brahmi based on immunoassay using a monoclonal antibody against bacopaside I. Phytochem Anal 2007; 18(5):411-418.
Pongkitwitoon B, Sakamoto S, Morinaga O, et al. Single-chain variable fragment antibody against ginsenoside Re as an effective tool for the determination of ginsenosides in various ginsengs. J Nat Med 2011; 65(1): 24-30.
Putalun W, Fukuda N, Tanaka H, Shoyama Y. A one-step immunochromatographic assay for detect-ing ginsenosides Rb1 and Rg1. Anal Bioanal Chem 2004a; 378(5):1338-1341.
Putalun W, Morinaga O, Tanaka H, Shoyama Y. Development of a one-step immunochromatographic strip test for the detection of sennosides A and B. Phytochem Anal 2004; 15(2):112-116.
Putalun W, Tanaka H, Shoyama Y. Rapid detection of glycyrrhizin by immunochromatographic assay.Phytochem Anal 2005; 16(5):370-4.
Putalun W, Tanaka H, Shoyama Y. Rapid separation of solasodine glycosides by an immunoaffinity column using anti-solamargine monoclonalantibody. Cytotechnology 1999; 31(1-2):153 158.
Putalun W, Tanaka H, Yahara S, Lhieochaiphan S, Shoyama Y. Survey of solasodine-type glycoalkaloids by western blotting and ELISA using anti-solamargine monoclonal antibody. Biol Pharm Bull 2000; 23(1):72-5.
Putalun W, Taura F, Qing W, Matsushita H, Tanaka H, Shoyama Y. Anti-solasodine glycosidesingle-chain Fv antibody stimulates biosynthesis of solasodine glycoside in plants. Plant Cell Rep 2003; 22(5):344-349.
Putalun W. Technology of compact MAb and its application for medicinal plant breeding named as missile type molecular breeding. Current Drug Discovery Technologies 2011; 8:24-31.
Sakamoto S, Putalun W, Tsuchihashi R, Morimoto S, Kinjo J, Tanaka H. Development of an enzyme-linked immunosorbent assay (ELISA) using highly-specific monoclonal antibodies against plumbagin. Anal ChimActa 2008; 607(1):100-105.
Sakamoto S, Taura F, Putalun W, et al. Construction and expression of specificity-improved single - chain variable fragments against the bioactive naphthoquinone, plumbagin. Biol Pharm Bull 2009; 32 (3): 434-439.
Sakamoto S, Taura F, Tsuchihashi R, et al. Expression, purification, and characterization of anti-plumbagin single-chain variable fragment antibody in Sf9 insect cell. Hybridomas 2010; 29(6):481-488.
Shan SJ, Tanaka H, Shoyama Y. Enzyme-linked immunosorbent assay for glycyrrhizin using anti-glycyrrhizin monoclonal antibody and an eastern blotting technique for glucuronides of glycyrrhetic acid. Anal Chem 2001; 73(24):5784-5790.
Shan SJ, Putalun W, Fukuda N, Morinaga O, Tanaka H, Shoyama Y. ELISA, Western blotting, immuno-cytolocalization and immunoaffinity column for naturally occuring bioactive compounds using monoclonal antibodies. J Food and Drugs Analysis 2000; 8(4): 258-269.
Shoyama Y, Fukada T, Murakami H. Production of monoclonal antibodies and ELISA for thebaine and codeine. Cytotechnology 1996; 19: 55-61.
Shoyama Y, Morinaga O, Putalun W, Juengwatanatrakul T, Tanaka H. New strategy for the analysis of traditional Chinese medicine using monoclonal antibodies. Proceeding of The 2nd Sino-Thai International Conference 2007a. 2-6th December 2007; 2: 35-39.
Shoyama Y. Monoclonal antibodies against small molecule natural products and their applications, Eastern blotting and knockout extract. Pharmaceuticals 2011; 4: 950-963.
Shoyama Y. Pharmacognosical study during 40 years. Yakugakuzasshi 2007; 127(10):1593-1620.
Sritularak B, Morinaga O, Yuan CS, Shoyama Y, Tanaka H. Quantitative analysis of ginsenosides Rb1, Rg1, and Re in American ginseng berry and flower samples by ELISA using monoclonal antibodies. J Nat Med 2009; 63(3):360-3.
Sritularuk B, Juengwatanatrakul T, PutalunW, Tanaka H, MorimotoS. A rapid one-step immuno-chromato- graphic assay for the detection of asiaticoside. J Nat Med 2011; DOI 10.1007/s11418-011-0582-2. [In press]
Tanaka H, Fukuda N, Shoyama Y. Eastern blotting and immunoaffinity concentration using monoclonal antibody for ginseng saponins in the field of traditional Chinese medicines. J Agric Food Chem 2007a; 55(10):3783-7.
Tanaka H, Putalun W, De-Eknamkul W, Matangkas-ombut O, Shoyama Y. Preparation of a novel monoclonal antibody against the antimalarial drugs, artemisinin and artesunate. Planta Med 2007b; 73(10):1127-1132.
Tanaka H, Putalun W, Tsuzaki C, Shoyama Y. A simple determination of steroidal alkaloid glycosides by thin-layer chromatography immunostaining using monoclonal antibody against solamargine. FEBS Lett 1997; 404(2-3): 279-82.
Tanaka H, Shoyama Y. Monoclonal antibody against tetrahydrocannabinolic acid distinguishes Cannabis sativa samples from different plant species. Forensic SciInt 1999; 106(3):135-46.
Tian M, Tanaka H, Shang M, et al. Production, characterization of a monoclonal antibody against aristolochic acid-II and development of its assay system. Am J Chin Med 2008; 36(2):425-36.
Tothiam C, Phrompittayarat W, Putalun W, et al. An enzyme-linked immunosorbant assay using monoclonal antibody against bacosideA(3) for determination of jujubogenin glycosides in Bacopamonnieri (L.) Wettst. Phytochem Anal 2011; DOI 10.1002/pca.1293. [In Press]
Uto T, Tuvshintogtokh I, Shoyama Y. Preparation of knockout extract for determination of really active compound using MAb. Current Drug Discovery Technologies 2011; 8:16-23.
Xu J, Tanaka H, Shoyama Y. One-step immunochroma- tographic separation and ELISA quantification of glycyrrhizin from traditional Chinese medicines. J Chromatogr B Analyt Technol Biomed Life Sci 2007; 850(1-2):53-8.
Xuan L, Tanaka H, Xu Y, Shoyama Y. Preparation of monoclonal antibody against crocin and its characterization. Cytotechnology 1999; 29(1):65-70.
Yanagihara H, Sakata R, Minami H, Tanaka H, Shoyama Y, Murakami H. Immunoaffinity column chromatography against forskolin using an anti-forskolin monoclonal antibody and its applications. Anal Chim Acta 1996 ; 335: 63 70.
Yanagihara H, Sakata R, Shoyama Y, Murakami H. Rapid analysis of small samples containing forskolin using monoclonal antibodies. Planta Med 1996 b; 62:169–172.
Yokota S, Onohara Y, Shoyama Y. Immunofluorescence and Immunoelectron Microscopic Localization of Medicinal Substance, Rb1, in Several Plant Parts of Panax ginseng. Current Drug Discov-ery Technologies 2011; 8:51-59.
Yuan CS, Tanaka H. Bioactivity of american ginseng by knockout extract preparation using monoclonal antibody. Current Drug Discovery Technolo-gies 2011; 8:32-41.
Zhu S, Shimokawa S, Tanaka H, Shoyama Y. Development of an assay system for saikosaponin a using anti-saikosaponin a monoclonal antibodies. Biol Pharm Bull 2004; 27(1):66-71.