Antioxidative and Cytotoxic Effect of the Extract from Camellia nitidissima Chi.

Main Article Content

Xu Zhaoa
Bungorn Sripanidkulchai
Khaetthareeya Sutthanut
Xiao-Xun Wanga

Abstract

Introduction: Camellia nitidissima Chi. leaves or yellow camellia is a traditional Chinese herb called “Jin Hua Cha” in Chinese medicine. It is commonly used for the treatment of liver diseases, faucitis, diarrhea, edema, hypertension, and tumor. This study aimed to investigate the antioxidative and cytotoxic effects of the extracts from Camellia nitidissima Chi. leaves. Materials and Method: Dried Camellia nitidissima Chi. leaves were extracted with 70% ethanol (F1), then further extracted with 40-60% petroleum ether (F2), ethyl acetate (F3), n-Butanol (F4) and water (F5) individually to obtain 5 fractions. The plant extracts were determined for antioxidative activity by DPPH assay, and cytotoxicity effect to HepG2 hepatoma cell line by MTT assay. Results: The results demonstrated the different potency of action among the different extracts. The F5 (water fraction) had notable anti-oxidative potential with EC50 of 63.31 ppm. Based on the lowest IC50 at 184.0 µg/ml of F4 (n -butanol fraction) this fraction should be further studied for anti-cancer activity. Conclusion: The antioxidant properties and cytotoxicity to a HepG2 hepatoma cell line of Camellia nitidissima Chi. leave extracts were studied. However, further studies should consider identifying related mechanisms, effcacy of action, and safety for use.

Downloads

Download data is not yet available.

Article Details

Section
Research Articles

References

Deng GY, Yang ZD, Lu TL. A brief review of research on yellow camellia in china. Guangxi Agric Biol Sci. 2000, 19:119–124.
Duan XX, Tang XF, Su JJ. Study on Inhibition of C. chrysantha on DEN induction of murine liver cancer. J Med Res, 2006, 35(6): 14-16.
Fu JY, Fu HJ, Fu X, et al. Hepu Golden Camellia (flower)–Investigation on chemical structure activity relationship for efficacy of tea polyphenols. Guilin: 2nd International symposium on Cammellia nitidissima, 2005.
Fu LG. China Plant Red Data Book, Science Press, Beijing, China, 1992, 648–649.
Huang XC. Addendum on the medicinal plants of Guangxi. Guangxi Sciences, 1999, 6(1): 75.
Kim SJ and Kim GH. Quantification of quercetin in different parts of onion and its DPPH radical scavenging and antibacterial activity. Food Sci Biotechnol, 2006, 15(1): 39–43.
Lee JH, Renita M, Pioritto RJ, St.Martin SK, Schwartz SJ, Vodovotz Y. Isoflavone characterization and antioxidant activity of Ohio soybeans. J Agri Food Chem, 2004, 52(9): 2647–2651.
Li CY, Duan XX, Su JJ. Impact of leaves and flowers of Camellia Chrysantha (Hu) Tuyama of different concentrations on diethyl nitrosamine induced precancerous lesion to liver of rat and hepatoma cells BEL-7404. J Guangxi Medical University, 2007, 24(5): 660-663.
Liang SY. Golden Camellias. China Forestry Press, Beijing. 1993.
Liu J, Shen HM, Ong CN. Salvia miltiorrhiza ingibits cell growth and induces apoptosis in human hepatoma Hep G2 cells. Cancer Letters, 2000, 153(1-2): 85-93.
Milardovic S and Ivekovic D. A novel amperometric method for antioxidant activity determination using DPPH free radical. Bioelectrochem. 2006, 68: 175–180.
Mossman BT. In vitro approaches for determining mechanisms of toxicity and carcinogenicity by asbestos in the gastrointestinal and respiratory tracts. Environ Health Perspect.1983, 53: 155–161.
Nishimoto SI, Hashimoto F, Shimizu K, and Sakata Y. Petal coloration of interspecific hydrids between Camellia chysantha × C. japonica. Jpn. Soc. Hort. Sci. 2004, 73: 189–191.
Parks CR. Breeding progress with yellow camellias. American Camellia Yearbook, 2000, 9–10.
Qin XM, Lin HG, Ning EC. Antioxidative properties of extracts from the leave of Camellia Chrysantha(Hu) Tuyama. Food Sci and Technol, 2008, 2: 189-191.
Sripanidkulchai B, Fangkrathok N, Junlatat J, Sripanidkulchai K. Antioxidative activity and antimutagenicity of three plants in Annonaceae in Plant Genetics Conservation at Khokphutaka, Amphur Phuwiang, Khon Kaen. IJPS. 2008, 4(7): 104-112.
Su ZM, and Mou XL. Geographic distribution of Camellia section Chrysantha from China. Guihaia. 1988, 8:75–81.
Wei X, Cao HL, Jiang YS, Ye WH, Ge XJ, Li F. Population genetic structure of Camellia nitidissima (Theaceae) and conservation implications. Bot Stud (Taipei, Taiwan). 2008, 49:147–153.
Wettasinghe M and Shahidi F. Scavenging of reactive-oxygen species and DPPH free radicals by extracts of borage and evening primrose meals. Food Chem, 2000, 70(1), 7–26.
Xu J, Chen S, Hu Q. Antioxidant activity of brown pigment and extracts from black sesame seed (Sesamum indicum L). Food Chem, 2005, 91(1): 79–83.
Yan DM and Yao AD. Antioxidant activity study on polyphenol of Camellia Chrysantha leaves. J Henan University of Technology (Natural Science Edition), 2009, 30(2): 43-45.
Yan YQ, Wang DL, Wang YF. Camellia nitidissima Chi. J Tea 2003, 29(2): 102-103.
Yang QH, Wei X, Zeng XL, Ye WH, Yin XJ, Wang ZM, Jiang YS. Seed biology and germination ecophysiology of Camellia nitidissima. For Ecol Manag. 2008, 255: 113–118.
Ye CX and Xu ZY. A Taxonomy of Camellia Sect. Chrysantha Chang. Acta Sci. Nat. U. Sunyatseni. 1992, 31: 68–77.