Antioxidative and Cytotoxic Effect of the Extract from Camellia nitidissima Chi.

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Xu Zhaoa
Bungorn Sripanidkulchai
Khaetthareeya Sutthanut
Xiao-Xun Wanga


Introduction: Camellia nitidissima Chi. leaves or yellow camellia is a traditional Chinese herb called “Jin Hua Cha” in Chinese medicine. It is commonly used for the treatment of liver diseases, faucitis, diarrhea, edema, hypertension, and tumor. This study aimed to investigate the antioxidative and cytotoxic effects of the extracts from Camellia nitidissima Chi. leaves. Materials and Method: Dried Camellia nitidissima Chi. leaves were extracted with 70% ethanol (F1), then further extracted with 40-60% petroleum ether (F2), ethyl acetate (F3), n-Butanol (F4) and water (F5) individually to obtain 5 fractions. The plant extracts were determined for antioxidative activity by DPPH assay, and cytotoxicity effect to HepG2 hepatoma cell line by MTT assay. Results: The results demonstrated the different potency of action among the different extracts. The F5 (water fraction) had notable anti-oxidative potential with EC50 of 63.31 ppm. Based on the lowest IC50 at 184.0 µg/ml of F4 (n -butanol fraction) this fraction should be further studied for anti-cancer activity. Conclusion: The antioxidant properties and cytotoxicity to a HepG2 hepatoma cell line of Camellia nitidissima Chi. leave extracts were studied. However, further studies should consider identifying related mechanisms, effcacy of action, and safety for use.


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