Ameliorative Effect of Apium graveolens L. on Scopolamine-Induced Amnesia Mice

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Boonruamkaew Phetcharat
Sukketsiri Wanida
Tanasawet Supita
Chonpathompikunlert Pennapa


Introduction: Apium graveolens L. has been claimed to possess therapeutic potential for central nervous system disorders. Thus, the present study aimed to evaluate its effect on scopolamin e-induced memory impairment in mice Methods: Forty-eight C57BL/6 mice were subjected to Morris water maze and object recognition test in order to determine the spatial and non-spatial memory. Additionally, the brain acetylcholinesterase (AChE) activities were also quantifi ed. Aricept was used as a positive control. Results: Oral administration of Apium graveolens L. extract (125, 250, 375 and 500 mg/kg BW for consecutive 28 days) signifi cantly decreased the escape latency time but increased the discrimination index when compared to vehicle (p<0.001) and aricept-treated groups (p<0.05). Furthermore, the AChE activities in cerebral cortex and hippocampus signifi cantly decreased in animals receiving 125, 250, 375 and 500 mg/kg BW of Apium graveolens L. extract (p<0.001) when compare to vehicle treated group and show a level similar to those observed in aricept treated group. Conclusion: These result suggested that Apium graveolens L. has benefi cial effects against scopolamine-induced amnesia by regulating cholinergic system and promoting memory enhancement via decreased activity of AChE.


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Ahmed T, Gilani AH. Inhibitory effect of curcuminoids on acetylcholinesterase activity and attenuation of scopolamine-induced amnesia may explain medicinal use of turmeric in Alzheimer’s disease. Pharmacol Biochem Behav 2009; 91(4): 554-559.

Al-Hindawi MK, Al-Deen IHS, Nabi MHA, Ismail MA. Anti-infl ammatory activity of some Iraqi plants using intact rats. J Ethnopharmacol 1989; 26(2): 163-168.

Al-Howiriny T, Alsheikh A, Alqasoumi S, Al-Yahya M, ElTahir K, Rafatullah S. Gastric antiulcer, antisecretory and cytoprotective properties of celery (Apium graveolens) in rats. Pharm Biol 2010; 48(7): 786-793.

Antunes M, Biala G. The novel object recognition memory: neurobiology, test procedure, and its modifications. Cogn Process 2012; 13: 93-110.

Baananou S, Piras A, Marongiu B, Dessì MA, Falconieri D, Porcedda S, Rosa A, Boughattas NA. Antiulcerogenic activity of Apium graveolens seeds oils isolated by supercritical CO2. Afr J PharmPharacol2012; 6(10): 756-762.

Branković S, Kitić D, Radenković M, VeljkovićS, Kostić M, Miladinović B, PavlovićD. Hypotensive and cardioinhibitory effects of the aqueous and ethanol extracts of celery (Apium Graveolens, Apiaceae). Acta Medica Medianae. 2010; 49(1): 13-16.

Čolović MB, Krstić DZ, Lazarević-Pašti TD, Bondžić AM, Vasić VM. Acetylcholinesterase Inhibitors: Pharmacology and Toxicology. Curr Neuropharmacol 2013; 11: 315-335.

Dhingra D, Kumar K. Memory-Enhancing Activity of Palmatine in Mice Using Elevated Plus Maze and Morris Water Maze. Adv Pharmacol Sci 2012: 1-7.

Doody RS, Geldmacher DS, Gordon B, Perdomo CA, Prattr D. Open-label, multicenter, phase 3 extension study of the safety and efficacy of donepezil in patients with Alzheimer disease. Arch Neurol 2001; 58: 427-433.

Ellman GL, Courtney KD, Andres VJr, Feather-Stone RM. A new and rapid colorimetric determination of acetylcholinesterase activity. Biochem Pharmacol 1961; 7: 88-95.

Feng X, Peng Y, Liu M, Cui Y. DL-3-n-butylphthalide extends survival by attenuating glial activation in a mouse model of amyotrophic lateral sclerosis. Neuropharmacology 2012; 62: 1004-1110.

Gholamhoseinian A, Moradi MN, Sharifi- far F. Screening the methanol extracts of some Iranian plants for acetylcholinesterase inhibitory activity. Res Pharm Sci 2009; 4(2): 105-112.

Gusman J, Malonne H, Atassi G. A reappraisal of the potential chemopreventive and chemotherapeutic properties of resveratrol.Carcinogenesis 2001; 22: 1111-1117.

Iswantini D, Ramdhani TH, Darusman LK. In vitro inhibition of celery (Apium graveolens L.) extract on the activity of Xanthine oxidase and determination of its active compound. Indo J Chem 2012; 12(3): 247-254.

Jain GC, Pareek H, Khajja BS, Jain K, Jhalani S, Agarwal S, Sharma S. Modulation of di-(2 ethylhexyl) phthalate induced hepatic toxicity by Apium graveolens L. seeds extract in rats. Afr J Biochem Res 2009; 3: 222-225.

Ko F, Huang T, Teng C. Vasodilatory action mechanisms of apigenin isolated from Apium graveolens in rat thoracic aorta. Biochem Biophys Acta 1991; 1115: 69-74.

Lahiri DK, Farlow MR, Sambamurti K, Greig N, Giacobini E, Schneider L. A critical analysis of new molecular targets and strategies for drug developments in Alzheimer’s disease. Curr Drug Targets 2003; 4: 97-112.

Lin Y, Shi R, Shen HM. Luteolin, a flavonoid with potentials for cancer prevention and therapy. Curr Cancer Drug Targets 2008; 8(7): 634-646.

Martin RF, Jeffrey LC. Effective pharmacologic management of Alzheimer’s disease. Am J Med 2007; 120: 388-397.

Misic D, Zizovic I, Stamenic M, Asanin R, Ristic M, Petrovic SD, Skala D. Antimicrobial activity of celery fruit isolates and SFE process modeling. Biochem Eng J 2008; 42: 148-152.

Momin RA, Nair MG. Mosquitocidal, nematicidal, and antifungal compounds from Apium graveolens L. seeds. J Agric Food Chem 2001; 49(1): 142-145.

Momin RA, Nair MG. Antioxidant, cyclooxygenase and topoisomerase inhibitory compounds from Apium graveolens Linn. seeds. Phytomedicine 2002; 9: 312-318.

Morris R. Developments of a water-maze procedure for studying spatial learning in the rat. J Neurosci Methods 1984; 11: 47-60.

Ovodova RG, Golovchenko VV, Popov SV, Popova GY, Paderin KM, Shashkov AS, Ovodov YS. Chemical composition and anti-inflammatory activity of pectic polysaccharide isolated from celery stalks. Food Chem 2009; 114: 610-615.

Park SY, Kim HS, Hong SS, Sul D, Hwang KW, Lee D. The neuroprotective effects of traditional oriental herbal medicines against β-amyloid-induced toxicity. Pharm Biol 2009; 47: 976-981.

Patel D, Shukla S, Gupta S. Apigenin and cancer chemoprevention: progress, potential and promise. Int J Oncol 2007; 30: 233-245.

Peng Y, Sun J, Hon S, Nylander AN, Xia W, Feng Y, Wang X, Lemere CA. L-3-n-Butyl-phthalide improves cognitive impairment and reduces amyloid-β in a transgenic model of Alzheimer’s disease. J Neurosci2010; 30(24): 8180-8189.

Petersen RC. Scopolamine induced learning failures in man. Psychopharmacol 1977: 52: 283-289.

PopoviÇ M, KaurinoviÇ B, TriviÇ S, DukiÇ NM, BursaÇ M. Effect of celery (Apium graveolens) extracts on some biochemical parameters of oxidative stress in mice treated with carbon tetrachloride. Phytotherapy 2006; 20(7): 531-537.

Scatena R, Martorana GE, Bottoni P, Botta G, Pastore P, Giardina B. An update on pharmacological approaches to neurodegenerative diseases. Expert Opin Investig Drugs 2007; 16(1): 59-72.

Sun XL, Ito H, Masuoka T, Kamei C, Hatano T. Effect of Polygala tenuifolia root extract on scopolamine-induced impairment of rat spatial cognition in an eight-arm radial maze task. Biol Pharm 2007; 30: 1727-1731.

Szwajgier D, Borowiec K. Screening for Cholinesterase inhibitors in selected fruits and vegetables. EJPAU 2012; 15(2): 1-15.

Terry AV Jr, Buccafusco JJ. The cholinergic hypothesis of age and Alzheimer’s disease-related cognitive deficits: recent challenges and their implications for novel drug development. J Pharmacol Exp Ther 2003; 306:821-827.

Tsi D, Tan BKH. Cardiovascular pharmacology of 3-n-butylphthalide in spontaneously hypertensive rats. Phytother Res 1997; 11(8): 576-582.

Wang Q, Sun LH, Jia W, Liu XM, Dang HX, Mai WL, Wang N, Steinmetz A, Wang YQ, Xu CJ. Comparison of ginsenosides Rgl and Rbl for their effects on improving scopolamine-induced learning and memory impairment in mice. Phytother Res 2010; 24: 1748-1754.

Wei A, Shibamoto T. Antioxidant activities and volatile constituents of various essential oils. J Agric FoodChem 2007; 55(5): 1737-1742.

Yao Y, Ren G. Effect of thermal treatment on phenolic composition and antioxidant activities of two celery cultivars. Food Sci Technol-LEB 2011; 44(1): 181-185.

Zhang L, Huang Y, Lu L, Wai MSM, Chan WM, Yew DT. Effects of DL-3-n-Butylphthalide on Vascular Dementia and Angiogenesis. Neurochem Res 2012; 37: 911-919.