Antiproliferative Effect of Raphanus sativus L. var. caudatus Alef in Human Breast MCF-7 and Human Lung SK-LU1 Adenocarcinoma Cell Lines
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Abstract
Introduction: This study was aimed to investigate the in vitro antiproliferative effect of Thai rat-tail radish—Raphanus sativus L. var. caudatus Alef or RS—the Thai indigenous plant in the same family as broccoli and cabbage. The isothiocyanates (ITCs) have been well-pronounced as the chemopreventive compound found in Brassicaceae plant. Methods: In this study, pod and stem of RS were extracted by dichloromethane via liquid-liquid extraction. Pure ITCs compounds—sulforaphane and sulforaphene—were used as the standards and positive controls. The crude extracts were inve stigated for their antiproliferation assay in the human lung cancer (SK-LU1) and human breast cancer (MCF-7) in comparable with the normal Vero cell line. Results: The results showed that only extract of RS pod showed antiproliferative effect. The SK-LU1 cells were most sensitive to sulforaphane (IC50 = 8.10±0.62 μg/ml), sulforaphene (IC50 = 17.05±0.18 μg/ml) and the RS pods extract (IC50 = 84.22±2.39 μg/ml) than those in MCF-7 cells. Conclusion: The present study indicated the potential of RS for further extended study of the chemopreventive effect of RS.
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