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Peels of two species of Citrus, Citrus aurantifolia (Christm.) and Citrus hystrix and rhizome of Alpinia galanga were selected for various extractions. Three extraction solvents: methanol, aqueous and dichloromethane were used in this study. The test methods were seed germination bioassay which focused on percent inhibition of seed germination, seedling length bioassay and shooting length bioassay that refl ected the inhibitory effects on root elongation and shoot length of seedling plants of lettuce seeds (Lactuca sativa L.) by suitable germination and growth rate as the representative for weeds in the test such as sensitivity, simplicity, low cost and suitability. The result showed that the 10 mg/ml of methanol extracts of Citrus hystrix had signifi cant inhibitory effects on germination of lettuce seeds and inhibition of root length and 10 mg/ml of dichloromethane extracts of Alpinia galanga had signifi cant inhibitory effects on inhibition of root and shoot length which could be utilized as bioherbicide for future weed control.
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