Enhancement Mulberroside A Production in Morus alba L. Cell Cultures by Calcium Alginate Immobilization and Elicitation

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Chadathorn Inyai
Orapin Udomsin
Jukrapun Komaikul
Hiroyuki Tanaka
Boonchoo Sritularak
Waraporn Putalun


Introduction: Mulberroside A is a major stilbene glycoside in root bark extract of Morus alba
L. (Moraceae). This compound has been used commercially as an active ingredient in whitening
cosmetics due to its anti-tyrosinase and anti-oxidant activities. To enhance mulberroside A production,
this study aimed to investigate effect of immobilization and elicitation on accumulation of mulberroside
A in cell cultures of M. alba. Methods: M. alba cell cultures were immobilized in calcium alginate beads
and cultivated in Murashige and Skoog (MS) liquid medium containing 0.1 mg/L thidiazuron and 1 mg/L
napthalene acetic acid. Then, culture beads were elicited with methyl jasmonate and yeast extract and
observed mulberroside A accumulation at 24, 48 and 72 hr after elicitation. Results: Cell immobiliz
ation in calcium alginate enhanced the production of mulberroside A over control (un-immobilization)
by 1.4-fold in 40 days and 9.4-fold in 120 days. Addition of 50 mg/mL yeast extract and 50 μM methyl
jasmonate in 40 days, M. alba cell immobilized cultures resulted in greatest mulberroside A accumul
ation by yielded 32.74 mg/g DW which is 2.4- and 1.9-fold higher than control and immobilized cells,
respectively. In all conditions, more than 60% of total mulberroside A production was released into
culture medium. Conclusion: The results may indicate that immobilization slowed the growth rate and
stimulated mulberroside A release of M. alba cell cultures. Moreover, the combination between
immobilization and elicitation showed a synergistic effect to enhance the production of mulberroside A.


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