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Introduction: Mulberroside A is a major stilbene glycoside in root bark extract of Morus alba
L. (Moraceae). This compound has been used commercially as an active ingredient in whitening
cosmetics due to its anti-tyrosinase and anti-oxidant activities. To enhance mulberroside A production,
this study aimed to investigate effect of immobilization and elicitation on accumulation of mulberroside
A in cell cultures of M. alba. Methods: M. alba cell cultures were immobilized in calcium alginate beads
and cultivated in Murashige and Skoog (MS) liquid medium containing 0.1 mg/L thidiazuron and 1 mg/L
napthalene acetic acid. Then, culture beads were elicited with methyl jasmonate and yeast extract and
observed mulberroside A accumulation at 24, 48 and 72 hr after elicitation. Results: Cell immobiliz
ation in calcium alginate enhanced the production of mulberroside A over control (un-immobilization)
by 1.4-fold in 40 days and 9.4-fold in 120 days. Addition of 50 mg/mL yeast extract and 50 μM methyl
jasmonate in 40 days, M. alba cell immobilized cultures resulted in greatest mulberroside A accumul
ation by yielded 32.74 mg/g DW which is 2.4- and 1.9-fold higher than control and immobilized cells,
respectively. In all conditions, more than 60% of total mulberroside A production was released into
culture medium. Conclusion: The results may indicate that immobilization slowed the growth rate and
stimulated mulberroside A release of M. alba cell cultures. Moreover, the combination between
immobilization and elicitation showed a synergistic effect to enhance the production of mulberroside A.
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Abdemajeed NA, Khdil OA, Danial EN. Immobilization technology for enhancing bio-products industry. African Journal of Biotechnology 2012; 11: 13528- 13539.
Butt MS, Nazir A, Sulkn MT. Morus alba L. nature’s functional tonic. Trend in Food Sceince & Technology 2008. 19(10): 105-112.
Brodelius P. The potential role of immobilization in plant cell technology. Trend in Biotechnology 1985; 3(11): 280-285.
Kamaraiah P, Ramakrishna SV, Raddama P, Kavi Kashov PB. Enhanced production of plumbagin in immobilized cells ofPlumbago rosea by elicitation and in situ adsorption. Journal of Biotechnology 2003; 101:181-187.
Kim JK, Kim M, Sho SG, Kim MK, Kim SW, Lim YH. Biotransformation of mulberroside A from Morus alba results in enhancement of tyrosinase inhibition. J Ind Microbial Biotechnol 2010; 37: 631-637.
Komaikul J, Kitisripanya T, Tanaka H, Sritularuk B, Putalun W. Development of anenzyme-linked immunosorbent assay for specifi c detection of mulberroside A in mulberry (Morus alba L.) using anti-mulberroside A polyclonal antibody. Food Anal 2014; Methods, 7: 58-63.
Komaikul J, Putalun W, Tanaka H, Sritularuk B, Kitisripanya T. Effect of elicitors on mulberroside A production in Morus albaroot cultures. GRC KKU 2013, 37: 1101-1106.
Sanchez-Sampedro MA, Fernandez-Terrago J, Corchete P. Yeast extract and methyl jasmonate-induced silymarin production in cell cultures of Silybum marianum (L.) Gaertn. Journal of Biotechnlogy 2005; 119: 60-69.
Villegas M, Leon R, Brodelius P. Effects of alginate and immobilization by entrapment in alginate on benzophenanthridine alkaloid production in cell suspension cultures of Eschschltizia califonica. Biotechnology letter 1999, 21: 49-55.
Zhou J, Li SX, Wang W, Guo X, Lu X, Yua X, et al. Variations in the levels of mulberroside A, oxyresveratrol, and resveratrol in mulberries in difference seasons and during growth. Hindawi 2013; 7: 1-7.