Antibacterial Activities Of Essential Oil From Zingiber Cassumnar Roxb. Against Serratia marcescens, Associated With Bacterial Keratitis
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Abstract
Introduction: Serratia marcescens (SM) is responsible for bacterial keratitis.The occurrence of antibiotics and disinfectants resistant - SM necessitate the discovery of new anti- SM agents from natural plants leads. Methods:The antibacterial activities of essential oils from 18 medicinal plants (Zingiber cassumunar Roxb.(A), Zingiber offi cinale(B), Curcuma aromatica Salisb. (C), Chromolaena odoratum (L.) (D), Amomum bifl orum Jack (E), Citrus maxima (Burm.f).Merr.(F), Syzygium aromaticum (L.) Merr.& L.M.Perry. (G), Citrus hystrix DC. (H), Kaempferia galanga L. (I), Alpinia galangal (Linn.) Swartz (J), Cocos nucifera L.(K), Curcuma xanthorrhiza Roxb (L), Mentha cordifolia Opiz. (M), Curcuma iners Blume (N), Eucalptus globulus Labill. (O), Litsea cubeba Pers(P)., Curcuma longa L. (Q), and Citrus reticulate Blanco (R)), against 5 strains of SM, obtaining from Department of Medical Sciences Thailand Culture Collection (DMST); SM DMST4228, SM DMST 4424, SM DMST 5100, SM DMST 8845 and SM DMST 8846) were performed by using agar diffusion method (20 mg/disc). The
minimum inhibitory concentrations (MICs) of the selected oil were determined by agar dilution method. The selected oil was investigated antibacterial potency comparable with 5 antibiotics (gentamicin:GEN, chloramphenicol:Cam, erythromycin:ERY, ampicillin:Amp, and tetracyclin:Tet) by using the agar
diffusion method. Results: Among 18 essential oils, the antibacterial effect against the 5 strains of SM
revealed that A (phlai oil) possessed signifi cantly the strongest antibacterial effi cacy with the range of its inhibition zones was 9.70-20.42 mm. The oil showed higher anti- SM activities against the 5 strains than AMP and ERY did. The best MIC value of A was 5 mg/mL. Conclusion:These fi ndings indicate the possibility to use phlai oil for the topical treatment of bacterial keratitis against SM.
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