Development of Immunoassay Using Monoclonal Antibody and Recombinant Antibody for Natural Products
Article Sidebar
Keywords:
Recombinant antibody ELISA Artemisinin
Main Article Content
Abstract
Introduction: Artemisinin (AM), which is a sesquiterpene lactone, is a well-known anti-mala rial compound isolated from Artemisia annua. We had prepared a monoclonal antibody (mAb 1C1) showing specifi city for AM and artesunate (AS), and we developed an indirect competitive enzymelinked immunosorbent assay (icELISA) using this novel mAb. Moreover, a recombinant antibody derived
from mAb 1C1 was prepared to overcome insuffi cient mAb production by hybridoma culture. Method: The recombinant antibodies, which were derived from a monoclonal antibody against AM and AS (mAb 1C1) prepared by us, were expressed by Escherichia coli cells, and their reactivity and specifi city were characterized. Results: As a result, the specifi city of the Fab was similar to that of mAb 1C1 in that it showed specifi c reactivity toward AM and AS only. The sensitivity of the icELISA (0.16 μg/mL – 40 μg/
mL for AM, 8.0 ng/mL – 60 ng/mL for AS) was suffi cient for analysis of anti-malarial drugs, and its utility for quality control of analysis of Artemisia spp. was validated. The Fab expression and refolding systems provided a good yield of high-quality antibodies.
from mAb 1C1 was prepared to overcome insuffi cient mAb production by hybridoma culture. Method: The recombinant antibodies, which were derived from a monoclonal antibody against AM and AS (mAb 1C1) prepared by us, were expressed by Escherichia coli cells, and their reactivity and specifi city were characterized. Results: As a result, the specifi city of the Fab was similar to that of mAb 1C1 in that it showed specifi c reactivity toward AM and AS only. The sensitivity of the icELISA (0.16 μg/mL – 40 μg/
mL for AM, 8.0 ng/mL – 60 ng/mL for AS) was suffi cient for analysis of anti-malarial drugs, and its utility for quality control of analysis of Artemisia spp. was validated. The Fab expression and refolding systems provided a good yield of high-quality antibodies.
Article Details
Section
Invited Speaker
In the case that some parts are used by others The author must Confirm that obtaining permission to use some of the original authors. And must attach evidence That the permission has been included