The effect of emulsifier system on mosquito repellent formulation from citronella oil

Main Article Content

Pichaya Worajuk
Phusanisa Thosathum
Warisada Sila-on
Utsansa Puapermpoonsiri


Introduction: This study aims to study the effect of emulsifier system for formulation development of mosquito repellent lotion containing citronella oil. Materials and Method: The homogenous emulsion was selected from two emulsifier systems such as Tween 80/Span 80 and Brij 72/Brij 721 with different concentration (3, 5, and 7 %) in order to perform stability study. As stability study, selected formulation would be kept at 45 OC 75% RH and at 25 OC for accelerated condition and ambient condition, respectively. Physicochemical and microbiological evaluation were conducted at 0, 1, 3, and 6 month. Results: Following 6 months of storage, it found that 1.54 % (w/w) Tween 80 and 1.46% (w/w) Span 80 provided the suitable formulations since the separation was not seen by centrifugation technique. In addition, color of formulation slightly altered from white to pale-yellow and the microorganism was not seen by total colony plate count technique. Conclusions: It is able to conclude that this formulation is extremely stable by using 1.54 %(w/w) Tween 80 and 1.46 %(w/w) Span 80 and keep this formulations at 25oC  more than 6 months of storage. However, the determination of active compound, efficacy of formulations and skin irritation test are required in further study


Download data is not yet available.

Article Details

Pharmaceutical Sciences


Gullapalli RP, Sheth BB. Influence of an optimized non-ionic emulsifier blend on properties of oil-in-water emulsions. Eur J Pharm Biopharm. 1999; 48(3): 233-8.

Jain A, Gautam SP, Gupta Y, Khambete H, Jain S. Development and characterization of ketoconazole emulgel for topical drug delivery. Der Pharmacia Sinica, 2010; 1(3): 221-231.

Jones TJ, Neustadter EL, Whittingham KP. Water-in-Crude Oil Emulsion Stability and Emulsion Destabilization by Chemical Demulsifiers. J Can Pet Technol 1978; 17(2): 1-10.

Khalil YI, Khasraghi AH, Mohammed EJ. Preparation and evaluation of physical and properties of clotrimazole emulgel. Iraqi J Pharm Sci 2011; 20(2): 19-27.

Kongkaew C, Sakunrag I, Chaiyakunapruk N, Tawatsin A. Effectiveness of citronella preparations in preventing mosquito bites: systematic review of controlled laboratory experimental studies. Trop Med Int Health 2011; 16(7): 802-10.

Krasantisuk S, Runnarong H. The development of skin care sericin lotion [The Bachelor Degree of Science in Pharmacy]. Nakhon pathom; Mahidol University; 2006.

Lv G, Wang F, Cai W, Zhang X. Characterization of the addition of lipophilic Span 80 to the hydrophilic Tween 80-stabilized emulsions. Colloids and Surfaces A: Physicochem. Eng. Aspects 2014; 447: 8-13.