Simple Flow Injection Spectrofluorimetric in Determination of Piroxicam

Abstract

A simple flow injection spectrofluorimetric method has been developed for the determination of piroxicam. The principle is based on the injection of a standard or sample solution into a moving, nonsegmented continuous carrier stream, which is then transported toward a spectrofluorometer detector. Method: The presented method was developed on the injection of 150 µl standard or sample solution of piroxicam into the carrier stream of 0.1 mol l^-1 nitric acid with the optimum flow rate at 4.0 ml  min^-1. The reaction is occurred in 100 cm of mixing coil length which was passed through to the fluorimetric detection unit. The fluorescence intensity was monitored using excitation and emission wavelength at 330 and 440 nm, respectively. Results: The calibration curve was established in concentration ranges 0.1-4.0 µg ml^-1 of piroxicam which was plotted against fluorescence intensity. For linearity within the optimum range, regression equation was shown y = 92.946x + 0.2335, and the coefficient of determination (r²) was 0.9994. The limit of detection (LOD) was 0.03 µg ml^-1. The percentage recoveries of proposed method had shown in range of 98.65%-100.48% and repeatability was found the relative standard deviation lesser than 0.43%. The effect of excipients which are usually added to pharmaceutical formulations was investigated on piroxicam analysis and shown none effected for determination of piroxicam. The difference between the results of proposed and reference methods was figured out statistically and showed no statistical difference at a 95% confidence level (n=10). Conclusion: The proposed method is fast and reasonably economic with the rate of 90 sample h^-1, and could be useful for routine analysis of piroxicam in capasules.