The Determination of Doxycycline Using Ferrous Sulfate Contained in Vitamin Tablets as Reagent
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Abstract
A simple spectrophotometric procedure for determining doxycycline is proposed. It is based on the reaction between this drug and ferrous sulfate from vitamin tablets in acidic solution. The presence of hydrogen peroxide enhances the absorption signal. Methods: The proposed method was applied successfully to analyze doxycycline in pharmaceutical dosage forms. The highest absorption was measured at 425 nm after mixing the solutions. Either standard or sample solution was mixed with 5.0 x 10-2 mol/L of ferrous sulfate from vitamin tablets in 5.0 x 10-3 mol/L of nitric acid and 2.5 x 10-2 % (v/v) of hydrogen peroxide in a ratio of 2.0 : 1.0 : 0.1 (v/v), respectively. The optimum conditions for determining doxycycline were investigated by the univariate method. Results: Using the proposed procedure under optimum conditions, linear calibration graphs were obtained for doxycycline concentrations from 1.0 to 50 µg/mL. Linear regression analysis of the absorbance of drug (y) versus drug concentration (x) yielded the equation y = 0.0042x + 0.0017. The correlation coefficient was 0.9994. The detection limit of doxycycline, the concentration of analyte that gave a signal different from the blank by an amount equal to three times the standard deviation of the blank signal (s/n=3), was found to be 0.3 µg/mL. The quantitation limit (defined as ten times the standard deviation of the blank signal) was 0.9 µg/mL. Moreover, the common excipients used as additives in the pharmaceutical dosage forms showed no effect on the proposed method. The results acquired by the proposed method compared favorably with those acquired by the reference method at a 95% confidence level with no significant difference (n=7). Conclusion: The proposed spectrophotometric method is a simple method for analysis of doxycycline with many advantages such as simple reagents, high accuracy, high reproducibility, and is therefore a rapid and acceptable alternative method for the routine quality control of doxycycline in drug formulations.
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