Surface Charge Effect of Furazolidone-loaded Polymeric Mixed Micelles for Delivery to Breast Cancer Cells (MCF-7) and Hepatocellular Carcinoma Cells (HepG2)
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Abstract
Nano drug delivery systems are important strategies to increase the specificity toward cancer cells as well as reduce the side effects of an administered drug. The purpose of this research was to study the effect of the charge of the polymeric micelle on the delivery of furazolidone into cancer cells. To be used as a guideline to develop anti-cancer pharmaceutical products with solubility problems. Material and Method: a drug delivery system was developed in the form of polymeric micelles prepared from the poloxamer 407 with Hexadecyltrimethylammonium bromide(CTAB) or lecithin, and loaded with furazolidone for delivery to breast cancer cells (MCF-7) and hepatocyte carcinoma (Hep G2) using a thin-film hydration method. Each formulation was prepared to compare the effect of the ions on the surface of two surfactants by considering the parameters of physical properties, particle size, particle size distribution and entrapment efficiency. Results: the results showed that optimal cationic and anionic formulations comprised the poloxamer 407: CTAB ratio of 200 mg:20 mg (particle size of about 307.14 ± 62.01 nm, zeta potential 22.36 ± 7.97 mV and entrapment efficiency 62.26 ± 1.49%), and the poloxamer 407: lecithin ratio of 200 mg:4 mg (particle size of about 311.42 ± 32.42 nm, zeta potential -22.34 ± 1.31 mV and entrapment efficiency 57.47 ± 3.14%). The selected formulations were then evaluated for cell cytotoxicity and cellular uptake of MCF-7 and Hep G2. Conclusion: From the experimental data, it was found that the surface charge of the drug delivery system is a factor that affects the treatment efficiency. Each type of cancer responds differently to the surface charge. For example, liver cancer cells have a higher rate of uptake of polar-substances than breast cancer cells. However, this study is a study of factors that affect the delivery of the substance to cancer cells. It may need to be tested in other types of cancer cells and will test toxicity with normal cells in the future.
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