Antioxidant Property of Aqueous Extracts from Leaf of Moringa oleifera Lam. and Pseuderanthemum palatiferum (Nees) Radlk.
Article Sidebar
Main Article Content
Abstract
Introduction: Moringa oleifera Lam. and Pseuderanthemum palatiferum (Nees) Radlk. are highly promoted in Thailand as miraculous plants with various properties. Based on their health-promoting property, the present study was conducted to investigate the antioxidant potential of the aqueous extracts of these plant species. Methods: Preliminary phytochemical examination indicated that the aqueous leaf extracts of M. oleifera and P. palatiferum contained cardiac glycoside, flavonoid, phenolic, reducing sugar, saponin, steroid, tannin and terpenoid. Moreover, anthraquinones and glycine were found only in M. oleifera and P. palatiferum leaf extract respectively. Results: Antioxidative efficiency study in vitro indicated that the extracts from M. oleifera and P. palatiferum were able to inhibit and scavenging reactive species (ABTS and DPPH) and also their level of total phenol and ascorbic acid were found to be equal. Beside the direct measurement of those antioxidants from the plant extracts, antioxidative activity in rats treated with the extracts from M. olerifera and P. palatiferum was also evaluated. Conclusion: It was shown that the serum and liver malondialdehyde (MDA) levels of rats treated with M. oleifera extract at 60, 120, 180 and 240 mg/kg BW and P. palatiferum extract at 5, 10, 15, 20 mg/kg BW were significantly lower than those of the controls (P<0.05). In contrast, superoxide dismutase (SOD) activities in the erythrocyte increased in all treated rat. It is therefore proposed that the aqueous extracts of M. oleifera and P. palatiferum contain potent antioxidant.
Article Details
In the case that some parts are used by others The author must Confirm that obtaining permission to use some of the original authors. And must attach evidence That the permission has been included
References
Adebayo AG, Akintoye HA, Olufolaji AO, et al. Assessment of organic amendments on vegetative development and nutrient uptake of Moringa olifera Lam. in the nursery. Asian J Plant Sci 2011; 10(1): 74-79.
Anila L, Vijayalakshmi NR. Antioxidant action of flavonoids from Mangifera indica and Emblica officinalis in hypercholesterolemic rats. Food Chem 2003; 83: 569–574.
Asada K. The water–water cycle in chloroplasts: scavenging of active oxygen and dissipation of excess photons. Annu Rev Plant Physiol Plant Mol Biol 1999; 50: 601–639.
Bac VH, Oanh, LTL. Amino acid and mineral contents in the leaf of Pseuderanthemum palatiferum (in Vietnamese with English summary). J Mater Sci Mater Med 2003; 8 : 11–15.
Baskin SI, Salem H. Oxidants, antioxidants and free radicals. CRC Press. Washington. DC, 1997.
Bode AM, Cunningham L, Rose RC. Spontaneous decay of oxidized ascorbic acid (dehydro- L-ascorbic acid) evaluated by high-pressure liquid chromatography. Clinical Chemical 1990; 36: 1807–1809.
Brand-Williams W, Bondet V, Berset C. Kinetics and mechanisms of antioxidant activity using the DPPH free radical method. Lebensm Wiss Technol 1997; 30: 609–615.
Buege JA, Aust SD. Microsomal lipid peroxidation. Methods Enzymol 1978; 52: 302–310.
Chumark P, Khunawat P, Sanvarinda Y, Phornchi rasilp S, Morales NP: The in vitro and ex vivo antioxidant properties, hypolipidaemic and antiatherosclerotic activities of water extract of Moringa oleifera Lam. leaves. J Ethnopharmacol 2008; 116: 439-446.
Cowan MM. Plant products as antimicrobial agents. Clin Microbiol Rev 1999; 12: 564-582.
Dekker PJ, Muller H, Rassow J, Pfanner N. Char¬acterization of the preprotein translocase of the outer mitochondrial membrane by blue native electrophoresis. Biol Chem 1996; 377: 535-538.
Dieu HK, Hoa TV. Efficacy of Pseuderanthemum palatiferum powder against diarrhea of piglets. Cantho University, Cantho 2003; pp. 1–6.
Dieu HK, Loc CB, Yamasaki S, Hirata Y. The ethnobotanical and botanical study on Pseuderanthemum palatiferum as a new medicinal plant in the Mekong Delta of Vietnam. JARQ 2005; 39(3): 191-196.
Dizdaroglu M, Karakaya AE. Advances in DNA damage and repair: oxygen radical effects, cellular protection and biological consequences. Springer. New York. 1999.
Daodee S, Panya T, Chansri N, Sripanidkulchai B. Study of some antioxidant to the amount of Quercetin in Marum Extract. IJPS 2013; 8 (1) :123-127.
Faizi S, Siddiqui BS, Saleem R, Aftab K, Shaheen F, Gilani AH. Hypotensive constituents from the pods of Moringa oleifera. Planta Med 1998; 64(3): 225-228.Ferguson PJ, Kurowska E, Freeman DJ, Chambers AF, Koropatnick DJ. A flavonoids fraction from cranberry extract inhibits proliferation of human tumor cell lines. J Nutr 2004; 134(6): 1529-1535.
Halliwel B, Gutteridge JMC. Free radicals in Biology and Medicine. Oxford Clarendon Press 2 nd edition. 1989; 416-494.
Kaur GJ, Arora DS. In vitro antibacterial activity of three plants belonging to the family Umbelliferae. Int J Antimicrob Agents 2008; 31: 393-395.
Kokate CK. Pharmacohnosy. 16th Edn., Nirali Prakasham, Mumbai, India; 2001.
Kumar T, Larokar YK, Iyer SK, Kumar A, Tripathi DK. Phytochemistry and pharmacological activities of Silybum marianum: A Review. Int J Pharm Phytopharmacol Res 2011; 1(3): 124-133.
Leiris JD. Biochemistry of free radical. Heart and Metabolism 2003; 19 : 40-44.
Manosroi A, Manosroi J. Determination of bioactive compounds in roots of different ages Pueraria mirifica airy shaw suvatabandhu and Butea superba, Roxb. from various locations in Thailand. [serial online] 2005 Mar. [cited 2104 Feb 19]; ISHS Acta Horticulturae 678. Available from:http://wwwlib.teiep.gr/images/stories/acta/Acta%20680/680_12.pdf
Mehta LK, Balaraman R, Amin AH, Bafna PA, Gulati OD. Effect of fruits of Moringa oleifera on lipid profile of normal and hypercholesterolaemic rabbits. J Ethnop¬harmacol 2003; 86(2-3) : 191-195.
Pamok S, Saenphet S, Vinitketkumnuen U, Saen¬phet K. Antiproliferative effect of Moringa oleifera Lam. and Pseuderanthemum palatiferum (Nees) Radlk. extract on the colon cancer cell. J Med Plants Res 2012; 6: 139-145.
Pari L, Kumar NA. Hepatoprotective activity of Moringa oleifera on antitubercular drug-induced liver damage in rats. J. of Med Food 2002; 5(3):171-177.
Pawana P, Panomket S. Hoan Ngoc activity in Streptozotocin-induced diabetic mice. IJPS 2011; 7(2) : 22-28
Phan MG, Ha VB, Phan TS. Study on anti-oxy¬dative activities and preliminary investi¬gation on antibacterial, antifungal of extracted fraction rich in flavonoides from leaves of Pseuderanthemum palatiferum (Nees) Radlk. TC khoa hoc va cong nghe 2005; 9(1): 9-12.
Rajalakshmi D, Narasimhan S. Food antioxidants : sources and method of evaluation. In Madhavi DL, Despande SS, Salunkhe DK. (eds.) : Food Antioxidants 1996; 6-64.
Re R, Pellegrini N, Proteggente A, et al. Antioxidant activity applying an improved ABTS radical cation decolorisation assay. Free Rad Biol Med 1999; 26: 1231-1237.
Saenphet K, Jirakittirat R, Saenphet K. Gastro-protective effects and antioxidant activities of Paederia pilifera Hook.f. root extract. Chiang Mai Journal of Sciences 2014; 41(5.1): 1121-1131.
Schlessier K, Harwat M, Bohm V, Bitsch R. Assessment of antioxidant activity by using different in vivo test methods. Free Radical Res 2002; 36: 177–187.
Singleton VL, Rossi JrJA. Colorimetry of total phenolics with phosphomolybdic-phos¬photungstic acid reagents. J Enol Vitic 1965;16(3): 144-158.
Sinha B, Singh BR, Singh RL et al. Oxidative DNA damage protective activity, antioxidant and anti-quorum sensing potentials of Moringa oleifera. Food Chem Toxicol 2011; 47 : 1109-1116.
Tachakittirungrod S. Free radical scavenging activity of some Thai medicinal plant extracts. Unpublished master’s thesis. Graduate School, Mahasarakham Uni¬versity, Thailand 2014.
Tansuwanwong S, Yamamoto H, Imai K, Vinitket¬kumnuen U. Antiproliferative effect on colon cancer cell lines by aqueous extract from the bark of Millingtonia hortensis. Chiang Mai Med J 2007; 46(2) : 61-66.
Tongmai T, Saenphet s, Saenphet K. Mutagenicity Test of Leaf Extract from Moringa oleifera Lam. and Pseuderanthemum palatiferum (Nees) Radlk. Proceedings of the 2nd MJU-Phrae National Research Conference, 2011 September 1 – 3; Phrae, Thailand.
Tepe B, Sokmen M, Akput HA, et al. Antioxidative activity of the essential oils of Thymus sipyleus subsp. sipyleus var. sipyleus and Thymus sipyleus subsp. sipyleus var. rosulans. J Food Eng 2005; 66: 447- 454.
Tiwari P, Kaur B, Kaur M, Kaur G, Kaur H. Phytochemical screening and extraction. (a review) Internationale Pharmaceutica Sciencia 2005; 1: 98-106.
Ushimaru T, Nakagawa T, Fujioka Y, et al. Transgenic arabidopsis plants expressing the rice dehydroascorbate reductase gene are resistant to salt stress. Plant Physiology 2006; 163: 1179–1184.
Xin Z, Waterman DF, Hemken RM, Harmon RJ. Effect of copper status on neutrophil function, superoxide dismutase, and copper distribution in steer. J Dairy Sci 1991; 74: 3078.