In Vitro Anti-Herpes Simplex Virus Activities of PienTze Huang
Article Sidebar
Main Article Content
Abstract
Introduction:PienTze Huang (PZH), is a popular traditional Chinese medicine formula used for treat liver diseases, cancers and inflammation. Little is known about any antiviral activity of PZH. PZH with anti-herpes simplex virus (HSV) activity has not been reported yet.The aim of this study was to investigate effects of PZH on HSV type 1 (HSV-1) and HSV type 2 (HSV-2) infectionsin vitro. Method: Modified plaque reduction assay was used to demonstrate the effectsof PZH on HSV-1, KOS strain and HSV-2, Baylor 186 strain, compared with those of acyclovir (ACV).Results:The 50 % inhibition concentrations (ICs50) of PZH were 297.31 ± 16.72 and 283.99 ± 28.93 µg/ml for HSV-1 and HSV-2, respectively. Whereas the IC50 of ACV were 0.21 ± 0.012 and 0.63 ± 0.18 µg/ml for HSV-1 and HSV-2, subsequently. Cytotoxicity test showed that the 50 % cytotoxic concentrations (CCs50) of PZH and ACV were 1,414.21 and 282.24 µg/ml, respectively. Moreover, thetherapeutic indexs (TIs) of PZH were 4.75 and4.98; and those of ACV were 1,340.48 and 451.82 for HSV-1 and HSV-2, subsequently. Conclusion: The results of this study were first revealed that PZH possess anti-HSV activitiesbut the TIs were slightly low. Consequently, PZH possesses anti-HSV activities but is not suitable for treatment patient with single HSV infection. PZH might be used for treatment patient with cancer and HSV infection. However, the further studies, in vivo and clinical trials are necessary for supporting our proposed treatment.
Article Details
In the case that some parts are used by others The author must Confirm that obtaining permission to use some of the original authors. And must attach evidence That the permission has been included
References
Ayoade FO, Todd JR. Herpes smplex [Online]. 2017 Mar 09 [cited 2017 Jul 25]. Available from: https://emedicine.medscape.com/article/218580-overview
Bacon TH, Levin MJ, Leary JJ, Sarisky RT and Sutton D. Herpes simplex virus resistance to acyclovir and penciclovir after two decades of antiviral therapy. Clin Microbiol Rev 2003; 16(1): 114-128.
Chao TC, Wu ML, Tsai WJ, Ger J and Deng JF. Acute hepatic injury and renal failure after ingestion of snake gallbladder (case report). Clin Toxicol 2006; 44: 387-390.
He F, Wu HN, Cai MY, et al. Inhibition of ovarian cancer cell proliferation by Pien Tze Huang via the AKT mTOR pathway. Oncol Lett 2014; 7: 2047-2052.
Lee KK, Kwong WH, Chau FT, Yew DT and Chan WY. Pien Tze Huang protects the liver against carbon tetrachloride-induced damage. Pharmacol Toxicol 2002; 91(4): 185-192.
Lü L, Wai Maria SM, David T, Mak Yew & Ying T. Pien Tze Huang, a composite Chinese traditional herbal extract, affects survival of neuroblastoma cells. Int J Neurosci 2009; 119(2): 255-262.
Pei Y, Du Q, Liao PY, et al. Notoginsenoside ST-4 inhibits virus penetration of herpes simplex virus in vitro. J Asian Nat Prod Res 2011; 13(6): 498-504.
Reed LJ and Muench H. A simple method of estimating fifty per cent endpoints. Am J Hyg 1938; 27: 493-497.
Shin YK, Cai GY, Weinberg A, Leary JJ and Levin MJ. Frequency of acyclovir-resistant herpes simplex virus in clinical specimens and laboratory isolates. J Clin Microbiol 2001; 39(3): 913-917.
Superti F, Ammendolia MG and Marchetti M. New advances in anti-HSV chemotherapy. Curr Med Chem 2008; 15(9): 900-911.
Wannawiboon, W. Cow-exalted animal. Thaihealthbook [online] 1987 May [cited 2018 May 29]. Available from: https://www.doctor.or.th/article/detail/4172
Wei L, Chen P, Chen Y, et al. Pien Tze Huang suppresses the stem-likeside population in colorectal cancer cells. Molec Med Rep 2014; 9(1): 261-266.
Wirotesangthong M and Rattanakiat S. Anti-herpes simplex virus type 2 activities of some Thai medicinal plants. Thai J Pharm Sci 2006; 30(1-2):19-27.
Xu L, Cao Y. Native musk and synthetic musk ketone strongly induced the growth repression and the apoptosis of cancer cells. BMC Complement Altern Med 2016; 16: 511. DOI 10.1186/s12906-016-1493-2
Yoosook C, Bunyapraphatsara N, Boonyakiat Y and Kantasuk C. Anti-herpes simplex virus activities of crude water extracts of Thai medicinal plants. Phytomed: Int J Phytother Phytopharmacol 2000; 6(6): 411-419.
Yoosook C, Panpisutchai Y, Chaichana S, Santisuk T and Reutrakul V. Evaluation of anti-HSV-2 activities of Barleria lupulina and Clinacanthus nutans. J Ethnopharmacol 1999; 67: 179-187.
Zhang L, Lam WP, Lü L, et al. Protective effects and potential mechanisms of Pien Tze Huang on cerebral chronic ischemia and hypertensive stroke. Chinese Med 2010; 5: 35-49.
Zhang L, Lam WP, Lü L, et al. How would composite traditional Chinese medicine protect the brain - an example of the composite formula “Pien Tze Huang”. Curr Med Chem 2011; 18: 3590-3594.
Zhang Y, Wang Q, Niu S, Liu J and Zhang L. Pien Tze Huang induces apoptosis in multidrugresistant U2OS/ADM cells via downregulation of Bcl2, survivin and P-gp and upregulation of Bax. Oncol Rep 2014; 31(2): 763-70.