Identification of Reo noi (Amomum uliginosum K.D.Koenig) Crude Drug using ITS1 DNA Marker of Nuclear DNA
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Abstract
Introduction: Reo noi is a crude drug derived from the ripe fruit of Amomum uliginosum K. D. Koenig. (Zingiberaceae). It is generally used for carminative, antiflatulence, antinausea and antivomiting purposes. This study was aimed to authenticate Reo noi crude drugs by using the ITS1 (internal transcribed spacer1) DNA Marker of nuclear DNA. Methods: Fifteen Reo noi crude drugs were authenticated specimens and collected from traditional drug stores. Macroscopic characteristic, ITS1 nucleotide sequence and volatile oil composition were determined. Results: The macroscopic characters of Reo noi crude drugs were aggregate fruit and globular in shape with 3 lobes. Seeds were brown color. They were shown 0.21-0.51 g on average weight and 8.7-9.7 mm on average diameter. The ITS1 nucleotide sequences of all samples were 180 bp in lengths and G+C contents were 57.22-60.56%. Major constituents of volatile oil were bornyl acetate and camphor. We found that authentication of Reo noi crude drugs by using ITS1 DNA marker revealed 4 adulterants from 11 commercial crud drug samples. Conclusion: Based on our results, the ITS1 DNA marker was proper to authenticate Reo noi crude drugs.
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