The chondroitin sulfate expression profile in human amniotic fluid cells: a time course study

Authors

  • Sirinda Aungsuchawan Department of Anatomy, Faculty of Medicine, Chiang Mai University, Thailand
  • Junjira Keawdee Department of Anatomy, Faculty of Medicine, Chiang Mai University, Thailand
  • Chaniporn Puaninta Department of Anatomy, Faculty of Medicine, Chiang Mai University, Thailand
  • Nathaporn Pangjaidee Department of Anatomy, Faculty of Medicine, Chiang Mai University, Thailand
  • Siriwan Ong-Chai Department of Biochemistry, Faculty of Medicine, Chiang Mai University, Thailand
  • Peraphan Pothacharoen Department of Biochemistry, Faculty of Medicine, Chiang Mai University, Thailand

Keywords:

Expression, chondroitin sulfate, WF6 epitope, human amniotic fluid cells

Abstract

Object The purpose of this study is to investigate the trend of expression of chondroitin sulfate epitope (WF6) in association with a continuous culture of hAFCs for a period of 30 days.

Methods The hAFCs were obtained from the amniotic fl uid of pregnant women at 18 weeks of gestation (n=5). The cells were cultured in an RPMI 1,640 medium containing 20% FCS, Amnio-MAX-C100 16%, 0.03 mg/mL ampicillin and 0.1 mg/mL streptomycin (sigma, Buchs, Switzerland). The expression of chondroitin sulfate epitope (WF6) was detected continuously using immunocytochemistry and signal fl uorescence evaluated with the use of Image-pro Plus version 6.0.

Results The levels of the mean area of the chondroitin sulfate WF6 was correlated with those of mean density. The fi rst cycle showed a gradual increase from day 0 to day 12 and then a continuous decrease from day 12 to day 18. In the second cycle the level increased from day 18 to day 27 and decreased after day 27 to day 30.

Conclusion A reasonable conclusion is that the expression of chondroitin sulphate WF6 epitope in hAFCs gradually increased and then decreased in a cyclical pattern.

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Published

2016-04-01

How to Cite

1.
Aungsuchawan S, Keawdee J, Puaninta C, Pangjaidee N, Ong-Chai S, Pothacharoen P. The chondroitin sulfate expression profile in human amniotic fluid cells: a time course study. BSCM [Internet]. 2016 Apr. 1 [cited 2024 May 3];55(2):49-55. Available from: https://he01.tci-thaijo.org/index.php/CMMJ-MedCMJ/article/view/87734

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Vol. 55 No. 2 (2016): April-June

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Original Article

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