Investigating the Role of Genetic Polymorphism in the Exon 3 of ATG16L1 Gene in T2DM Patients: A Pilot Study

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Published: Jun 23, 2025
Keywords:
ATG16L1 HRM SNP T2DM Autophagy

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Haider Alnaji
1Medical Laboratory Department, Kufa Technical Institute, Al-Furat Al-Awast Technical University, Iraq
https://orcid.org/0000-0002-6977-2928
Haider Abbas Hadi Al-Mhanaa
Laboratories Techniques Department, College of Health and Medical Techniques, Al-Mustaqbal University, Iraq
https://orcid.org/0009-0001-2460-0567

Abstract

Objective  Diabetes mellitus (DM) is a metabolic disease characterized by persistently high blood sugar levels and has several potential causes.  We aimed to find out whether there are any single nucleotide polymorphisms in a particular region of the ATG16L1 gene which are linked to type 2 diabetes.


Methods  A total of forty participants were included in this study; 20 were part of the patient group and had type 2 diabetes mellitus (T2DM), while 20 were part of the healthy control group. High-resolution melt (HRM) analysis was used for genotyping along with sequencing for confirmation of the results.


Results The melting behavior showed consistent DNA sequence in the exon 3 of the ATG16L1 gene in both the patient and the healthy control groups in the study.


Conclusions The ATG16L1 gene does not include any SNPs in the exon 3 region associated with type 2 diabetes. However, further studies are needed to determine the role of the autophagy gene in T2DM. 

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How to Cite
1.
Alnaji H, Al-Mhanaa H. Investigating the Role of Genetic Polymorphism in the Exon 3 of ATG16L1 Gene in T2DM Patients: A Pilot Study. BSCM [internet]. 2025 Jun. 23 [cited 2025 Dec. 27];64(3):212-6. available from: https://he01.tci-thaijo.org/index.php/CMMJ-MedCMJ/article/view/275431
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Vol. 64 No. 3 (2025): July-September
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Original Article
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This work is licensed under a Creative Commons Attribution 4.0 International License.

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