The Detection of Touch DNA on Wooden Knife Handle Used as a Stabbing Weapon for Personal Identification.

Authors

  • เมธี บุญพิมพ์ โรงพยาบาลชัยภูมิ
  • Chaturong Kanchai Dep. of Forensic Medicine, Faculty of Medicine, Chiang Mai University
  • Tanin Bhoophat Dep. of Forensic Medicine, Faculty of Medicine, Chiang Mai University

Keywords:

Touch DNA, Y-STRs, Transfer, knife

Abstract

Background : DNA profiles from touched objects have been analyzed for many years. The purpose of this study was to examine the effectiveness of Y-STRs profiling of touch DNA from a wooden-handle knife used as a stabbing weapon. A group of 60 male volunteers were selected and asked to stab a foam box with a wooden-handle knife by holding it firmly. DNA from surface of the knife handles was extracted using a Qiagen® QIAamp DNA Investigator kit and amplified using a modified method of Investigator® Argus Y-12 QS protocol.

Result : The results of touch DNA profiling demonstrated that 6 of 60 (10% full profiles from all samples profiles) samples gave a full profile and 21 samples showed useful partial profiles (35% from all sample profiles). No significant difference was shown between right and left hand dominant (p = 0.5615 95% CI = -24.6237 to 44.1160, Chi-squared test of proportion).

Conclusion : This study demonstrated Y-STRs as touch DNA can be used in conjunction with personal identification.

Downloads

Download data is not yet available.

Author Biographies

Chaturong Kanchai, Dep. of Forensic Medicine, Faculty of Medicine, Chiang Mai University

assistant professor in Dep. of Forensic Medicine, Faculty of Medicine, Chiang Mai University

 

Tanin Bhoophat, Dep. of Forensic Medicine, Faculty of Medicine, Chiang Mai University

Professor in Dep. of Forensic Medicine, Faculty of Medicine, Chiang Mai University.

References

1. R.A.H. van Oorschot, M.K. Jones (1997) DNA fingerprints from fingerprints. Nature 387:767.
2. C.E.V. French, C.G. Jensen, S.K. Vintiner, D.A. Elliot, S.R. McGlashan (2008) A novel histological technique for distinguishing between epithelial cells in forensic casework. Forensic Science International 178:1-6.
3. R.A. Wickenheiser (2002) Trace DNA: a review, discussion of theory, and application of the transfer of trace quantities of DNA through skin contact. Jounal of Forensic Science 47(3):442-450.
4. A. Lowe, C. Murray, J. Whitaker, et al (2002) The propensity of individuals to deposit DNA and secondary transfer of low level DNA from individuals to inert surfaces. Forensic Science International 129:25-34.
5. G. Meakin, A. Jamieson (2013) DNA transfer: review and implications for casework. Forensic Science International Genetics 7:434-443.
6. D.J. Daly, C. Murphy, S.D. McDermott (2012) The transfer of touch DNA from hands to glass, fabric and wood. Forensic Science International Genetics 6:41-46.
7. Kayser M. (2017) Forensic use of Y-chromosome DNA: a general overview. Human Genetics 136(5):621-635.
8. Andrew McDonald, Emma Jones , Jennie Lewis , Paula O’Rourke (2015) Y-STR analysis of digital and/or penile penetration cases with no detected spermatozoa. Forensic Science International Genetics 15:84-89.
9. B.C.M. Pang, B.K.K Cheung 2007 Double swab technique for collecting touched evidence. Legal Medicine 9(4):181-184.
10. R.K. Farmen, R. Jaghø, P. Cortez, et al 2008 Assessment of individual shedder status and implication for secondary DNA transfer. Forensic Science International Genetics 1:415-417.
11. Stephen C.Y. Ip , Sze-wah Lin, Kam-ming Lai 2015 An evaluation of the performance of five extraction methods: Chelex® 100, QIAamp® DNA Blood Mini Kit, QIAamp® DNA Investigator Kit, QIAsymphony® DNA Investigator® Kit and DNA IQ™. Science and Justice 55(3):200-208.
12. QIAamp® DNA Investigator Handbook. https://b2b.qiagen.com/us/resources/resourcedetail?id=dcc5a995-3743-4219-914d-94d6a28e49b3&lang=en. Accessed 6 March 2017.
13. Investigator® Argus Y-12 QS Kit Handbook. http://algimed.by/download/Investigator-Argus-Y-12-QS-Handbook.pdf. Accessed 6 March 2017.
14. Gusmão, J.M., et al 2006 DNA Commission of the International Society of Forensic Genetics (ISFG): an update of the recommendations on the use of Y-STRs in forensic analysis. Forensic Science International Genetics 157: 187-197.
15. Tsuji A, Ishiko A, et al 2001 Personal identification using Y-Chromosomal short tandem repeats from bodily fluids mixed with semen. The American Journal of Forensic Medicine and Pathology 22(3):288-291.

Published

2020-11-02

How to Cite

1.
บุญพิมพ์ เ, Kanchai C, Bhoophat T. The Detection of Touch DNA on Wooden Knife Handle Used as a Stabbing Weapon for Personal Identification. Chiang Mai Med J. [Internet]. 2020 Nov. 2 [cited 2022 Jun. 29];59(3):121-5. Available from: https://he01.tci-thaijo.org/index.php/CMMJ-MedCMJ/article/view/239895

Issue

Section

Original Article