The Detection of Touch DNA on Wooden Knife Handle Used as a Stabbing Weapon for Personal Identification.
Keywords:Touch DNA, Y-STRs, Transfer, knife
Background : DNA profiles from touched objects have been analyzed for many years. The purpose of this study was to examine the effectiveness of Y-STRs profiling of touch DNA from a wooden-handle knife used as a stabbing weapon. A group of 60 male volunteers were selected and asked to stab a foam box with a wooden-handle knife by holding it firmly. DNA from surface of the knife handles was extracted using a Qiagen® QIAamp DNA Investigator kit and amplified using a modified method of Investigator® Argus Y-12 QS protocol.
Result : The results of touch DNA profiling demonstrated that 6 of 60 (10% full profiles from all samples profiles) samples gave a full profile and 21 samples showed useful partial profiles (35% from all sample profiles). No significant difference was shown between right and left hand dominant (p = 0.5615 95% CI = -24.6237 to 44.1160, Chi-squared test of proportion).
Conclusion : This study demonstrated Y-STRs as touch DNA can be used in conjunction with personal identification.
2. C.E.V. French, C.G. Jensen, S.K. Vintiner, D.A. Elliot, S.R. McGlashan (2008) A novel histological technique for distinguishing between epithelial cells in forensic casework. Forensic Science International 178:1-6.
3. R.A. Wickenheiser (2002) Trace DNA: a review, discussion of theory, and application of the transfer of trace quantities of DNA through skin contact. Jounal of Forensic Science 47(3):442-450.
4. A. Lowe, C. Murray, J. Whitaker, et al (2002) The propensity of individuals to deposit DNA and secondary transfer of low level DNA from individuals to inert surfaces. Forensic Science International 129:25-34.
5. G. Meakin, A. Jamieson (2013) DNA transfer: review and implications for casework. Forensic Science International Genetics 7:434-443.
6. D.J. Daly, C. Murphy, S.D. McDermott (2012) The transfer of touch DNA from hands to glass, fabric and wood. Forensic Science International Genetics 6:41-46.
7. Kayser M. (2017) Forensic use of Y-chromosome DNA: a general overview. Human Genetics 136(5):621-635.
8. Andrew McDonald, Emma Jones , Jennie Lewis , Paula O’Rourke (2015) Y-STR analysis of digital and/or penile penetration cases with no detected spermatozoa. Forensic Science International Genetics 15:84-89.
9. B.C.M. Pang, B.K.K Cheung 2007 Double swab technique for collecting touched evidence. Legal Medicine 9(4):181-184.
10. R.K. Farmen, R. Jaghø, P. Cortez, et al 2008 Assessment of individual shedder status and implication for secondary DNA transfer. Forensic Science International Genetics 1:415-417.
11. Stephen C.Y. Ip , Sze-wah Lin, Kam-ming Lai 2015 An evaluation of the performance of five extraction methods: Chelex® 100, QIAamp® DNA Blood Mini Kit, QIAamp® DNA Investigator Kit, QIAsymphony® DNA Investigator® Kit and DNA IQ™. Science and Justice 55(3):200-208.
12. QIAamp® DNA Investigator Handbook. https://b2b.qiagen.com/us/resources/resourcedetail?id=dcc5a995-3743-4219-914d-94d6a28e49b3&lang=en. Accessed 6 March 2017.
13. Investigator® Argus Y-12 QS Kit Handbook. http://algimed.by/download/Investigator-Argus-Y-12-QS-Handbook.pdf. Accessed 6 March 2017.
14. Gusmão, J.M., et al 2006 DNA Commission of the International Society of Forensic Genetics (ISFG): an update of the recommendations on the use of Y-STRs in forensic analysis. Forensic Science International Genetics 157: 187-197.
15. Tsuji A, Ishiko A, et al 2001 Personal identification using Y-Chromosomal short tandem repeats from bodily fluids mixed with semen. The American Journal of Forensic Medicine and Pathology 22(3):288-291.