Antioxidant activity and phytochemical of agricultural waste from Cannabis sativa

Authors

  • Panupan Sripan Division of Cannabis Health Science, College of Allied Health Sciences, Suan Sunandha Rajabhat University
  • Supanut Chankerd 2Bachelor’s student of Cannabis Health Sciences program, College of Allied Health Sciences, Suan Sunandha Rajabhat University
  • Rachata Kitiyanasup Bachelor’s student of Cannabis Health Sciences program, College of Allied Health Sciences, Suan Sunandha Rajabhat University
  • Palapon Chimpalee Bachelor’s student of Cannabis Health Sciences program, College of Allied Health Sciences, Suan Sunandha Rajabhat University
  • Sittichok Wongsonthom Bachelor’s student of Cannabis Health Sciences program, College of Allied Health Sciences, Suan Sunandha Rajabhat University
  • Suwadee Chokchaisiri Division of Cannabis Health Sciences, College of Allied Health Sciences, Suan Sunandha Rajabhat University
  • Rumrada Meeboonya 1Division of Cannabis Health Sciences, College of Allied Health Sciences, Suan Sunandha Rajabhat University
  • Orawan Wonganan 1Division of Cannabis Health Sciences, College of Allied Health Sciences, Suan Sunandha Rajabhat University
  • Chawalit Yongram 1Division of Cannabis Health Sciences, College of Allied Health Sciences, Suan Sunandha Rajabhat University
  • Anuvat Roongpisuthipong 1Division of Cannabis Health Sciences, College of Allied Health Sciences, Suan Sunandha Rajabhat University

Keywords:

Cannabis sativa, phytochemical, antioxidant activity, agricultural waste, gas chromatography mass spectrometry

Abstract

Oxidative stress is a case of several diseases such as cancer, Alzheimer's disease, neuroinflammation and neurodegenerative diseases. The plant is an antioxidant agent that can inhibit the oxidative stress. Therefore, this study aimed to analyze the chemical composition and evaluate the antioxidant activity of waste parts from the Peanut Butter Breath strain, leaves, twig, roots, and stems. The results showed that cannabis leaves yielded the highest extract at 6.37% and exhibited the strongest antioxidant activity using DPPH and ABTS assays, with the IC50 values of 149.81 µg/mL and 39.06 µg/mL, respectively. Moreover, cannabis leaves contained the highest total phenolic compounds and total flavonoids with values of 83.44 mg GAE/g extract and 75.79 mg QE/g extract, respectively. Chemical composition analysis using gas chromatography mass spectrometry (GCMS) revealed a diversity of compounds in different plant parts. High levels of tetrahydrocannabinol (THC) and cannabinol (CBN) were found in leaves, while roots contained high amounts of triterpenes and sterols such as friedelan-3-one and friedelanol. This study highlights the potential of cannabis waste, particularly leaves, for development into antioxidant-rich products with possible applications in the pharmaceutical, cosmetic, and dietary supplement industries. Furthermore, utilizing cannabis waste aligns with the circular economy concept, adding value to agricultural byproducts and reducing environmental impact. However, further research on the safety and efficacy of extracts from various cannabis parts in real-world applications is necessary to develop high-quality and safe products for consumers.

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Published

2025-04-30

How to Cite

1.
Sripan P, Chankerd S, Kitiyanasup R, Chimpalee P, Wongsonthom S, Chokchaisiri S, Meeboonya R, Wonganan O, Yongram C, Roongpisuthipong A. Antioxidant activity and phytochemical of agricultural waste from Cannabis sativa. J Med Health Sci [internet]. 2025 Apr. 30 [cited 2026 Jan. 16];32(1):18-33. available from: https://he01.tci-thaijo.org/index.php/jmhs/article/view/274080

Issue

Vol. 32 No. 1 (2025): เมษายน 2568

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Original article (บทความวิจัย)

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