The Antioxidant and biological activity of Dillenia parviflora Griff. Fruits
Keywords:
Dillenia parviflora Griff, Antioxidant activity, elastase, tyrosinase, hyaluronicAbstract
This study aimed to evaluate the antioxidant and biological activities of Dillenia parviflora Griff. (Dp) extract through various analyses, including determination of total phenolic content, DPPH radical scavenging assay for antioxidant activity, and inhibition of elastase, tyrosinase, and hyaluronidase enzymes. The study also assessed the inhibition of nitric oxide production in lipopolysaccharide (LPS)-stimulated macrophage (RAW 264.7) cells and evaluated the wound healing efficacy in human keratinocyte (HaCaT) cells. The results showed that the average total phenolic content in Dp was 28.14±0.97 mg GAE/g. Dp exhibited significantly lower compared to vitamin C (p = 0.019). A concentration 1 mg/mL of Dp demonstrated anti-inflammatory effects in RAW 264.7 cells, which were significantly less potent than triamcinolone acetonide (p = 0.003). It has an inhibitory effect on tyrosinase IC50 >1000 (mg/ml) less than vitamin C. Dp also showed slight inhibition of elastase activities, with the latter being significantly weaker than that of EGCG (p = 0.016). However, Dp did not inhibit hyaluronidase activity. The wound healing assay indicated that 1 mg/mL Dp could stimulate human skin cell migration faster than the control but slower than vitamin C (p = 0.49). Future studies should focus on identifying the active compounds responsible for these effects and conducting clinical trials to explore the potential application to herbal innovations.
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