Mechanisms of vasorelaxation induced by Quercetin from Anaxagorea luzonensis A. Grey in the rat aorta

Authors

  • Patcharin Tep-Areenan Department of Physiology, Faculty of Medicine, Srinakharinwirot University
  • Pattara Thiraphibundet Center of Excellence in Natural Products Chemistry, Department of Chemistry, Faculty of Science, Chulalongkorn University

Keywords:

Anaxagorea luzonensis, quercetin, vasorelaxation, endothelium, K channels, Ca2 influx

Abstract

Abstract
The aim of the present study was to study the vasorelaxant effects of quercetin from Anaxagorea luzonensis and its underlying mechanisms in the aortas of rats. Quercetin (1-100 µM) induced concentration-dependent vasorelaxations were reduced by endothelial denudation, and 300 µM NG-nitro-L-arginine methyl ester (L-NAME), but not 10 µM indomethacin. After raising the extracellular KCl concentration to 60 mM inhibited vasorelaxant responses to quercetin. Moreover, the responses to quercetin were inhibited by 5 mM tetraethyammonium, 30 µM barium chloride, and 10 µM glibenclamide, but not 1 mM 4-aminopyridine. Pre-incubation with quercetin (1-100 µM) inhibited the contractions induced by CaCl2 and methoxamine. The present findings demonstrated that in the rat isolated aorta, that vasorelaxant responses to quercetin were mediated in part via Nitric Oxide dependent pathways. Moreover, the activation of KIR, K Ca, K ATP channels seemed to play a role in quercetin-induced responses. Interestingly, the inhibition of extracellular Ca2+ influx is largely involved in the action of quercetin.

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Published

2021-12-30

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Original Article (บทความวิจัย)