Prebiotic and antioxidant potential of feruloyl-polysaccharide from rice straw digested by recombinant xylanase enzyme of Streptomyces sp. SWU10
Keywords:
prebiotics, antioxidant, feruloyl-polysaccharide, rice straw, Streptomyces sp. SWU10, xylanaseAbstract
Prebiotics and antioxidants are important substances for promoting health. In the present study, the products from degradation of non-pretreatment rice straw by recombinant xylanase of Streptomyces sp. SWU10 (rXynSW3) was investigated for their prebiotic and antioxidant activities. The rice straw was ground to powder, dissolved in phosphate buffer solution pH 6.0 before digesting by rXynSW3. The enzymatic products were analyzed by HPLC and HPAEC. The prebiotics property was evaluated by fermentation of the enzymatic products with probiotic strains including Lactobacillus plantarum F33 and Bifidobacterium adolescentis JCM1275 and pathogenic bacterial strains such as Bacteroides vulgatus JCM5826 and Clostridium hiranonis JCM10541. In vitro antioxidant activity of the enzymatic products was determined by DPPH assay. The HPLC and HPAEC analysis revealed that the major product was feruloyl-polysaccharide. The antioxidant activity of the enzymatic products determined by DPPH assay showed the IC 50 at 611.10 µg/mL compared to 494.72 µg/mL of ascorbic acid which was used as standard. The enzymatic products promoted growth of both probiotic strains but no effect on growth of pathogenic bacteria. In conclusion, the in vitro studies revealed that the feruloyl-polysaccharide the main product from non-pretreatment rice straw digested by rXynSW3 have potential of antioxidant and prebiotic activities.
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