Prebiotic and antioxidant potential of feruloyl-polysaccharide from rice straw digested by recombinant xylanase enzyme of Streptomyces sp. SWU10
Keywords:
prebiotics, antioxidant, feruloyl-polysaccharide, rice straw, Streptomyces sp. SWU10, xylanaseAbstract
Prebiotics and antioxidants are important substances for promoting health. In the present study, the products from degradation of non-pretreatment rice straw by recombinant xylanase of Streptomyces sp. SWU10 (rXynSW3) was investigated for their prebiotic and antioxidant activities. The rice straw was ground to powder, dissolved in phosphate buffer solution pH 6.0 before digesting by rXynSW3. The enzymatic products were analyzed by HPLC and HPAEC. The prebiotics property was evaluated by fermentation of the enzymatic products with probiotic strains including Lactobacillus plantarum F33 and Bifidobacterium adolescentis JCM1275 and pathogenic bacterial strains such as Bacteroides vulgatus JCM5826 and Clostridium hiranonis JCM10541. In vitro antioxidant activity of the enzymatic products was determined by DPPH assay. The HPLC and HPAEC analysis revealed that the major product was feruloyl-polysaccharide. The antioxidant activity of the enzymatic products determined by DPPH assay showed the IC 50 at 611.10 µg/mL compared to 494.72 µg/mL of ascorbic acid which was used as standard. The enzymatic products promoted growth of both probiotic strains but no effect on growth of pathogenic bacteria. In conclusion, the in vitro studies revealed that the feruloyl-polysaccharide the main product from non-pretreatment rice straw digested by rXynSW3 have potential of antioxidant and prebiotic activities.
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lignocellulosic waste: An alternative to
unravel the future bioenergy. Biofuels:
greenhouse gas mitigation and global
warming. 2018:291-305.
2. Kucharska K, Rybarczyk P, Holowacz I, et
al. Pretreatment of lignocellulosic
materials as substrates for fermentation
processes. Molecules 2018;23:2937.
doi:10.3390/molecules23112937.
3. Saini JK, Saini R, Tewari L. Lignocellulosic
agriculture wastes as biomass feedstocks
for second-generation bioethanol
production: concepts and recent
developments. Biotech 2015;5(4):337-53.
4. Taherzadeh MJ, Karimi K. Pretreatment of
lignocellulosic wastes to improve ethanol
and biogas production: A review. Int J Mol
Sci 2008;9(9):1621-51.
5. Day RL, Harpeer AJ, Woods RM, et al.
Probiotics: current landscape and future
horizons. Future Sci OA 2019;5(4):FSO391.
6. Wichienchot S, Thammarutwasik P,
Jongjareonrak A, et al. Extraction and
analysis of prebiotics from selected plants
from southern Thailand. Songklanakarin J
Sci Technol 2011;33(5):517-23.
7. M.T. Holtzapple. Hemicelluloses. Academic
Press 2003:3060-71.
8. Lin SH, Chou LM, Chien YW, et al. Prebiotic
effects of xylooligosaccharides on the
improvement of microbiota balance in
human subjects. Gastroenterol Res Pract
2016:5789232.
9. Jain I, Kumar V, Satyanarayana T.
Xylooligosaccharides: an economical
prebiotic from agroresidues and their
health benefits. Indian J Exp Biol
2015;53(3):131-42.
10. Christensen EG, Licht TR, Leser TD, et al.
Dietary xylo-oligosaccharide stimulates
intestinal bifidobacteria and lactobacilli
but has limited effect on intestinal
integrity rats. BMC Res Notes 2014;7:660.
11. Yu X, Yin J, Li L, et al. Prebiotic potential
of xylooligosaccharides derived from corn
cobs and their in vitro antioxidant activity
when combined with Lactobacillus.
J Microbiol Biotechnol 2015;25(7):1084-92.
12. Geetha K and Gunasekaran P. Purification
of endoxylanase from Bacillus pumilus
B20 for production of prebiotic
xylooligosaccharide syrup; An in vitro
study. Iran J Biotechnol 2017;15(4):232-40.
13. Yang J, Tang Q, Xu L, et al. Combining
of transcriptome and metabolome
analyses for understanding the utilization
and metabolic pathways of Xylooligosaccharide in Bifidobacterium
adolescentis ATCC 15703. Food Sci Nutr
2019;7(11):3480-93.
14. Van de Abbeele P, Marzorati M, Derde M,
et al. Arabinoxylans, inulin and
Lactobacillus reuteri 1063 repress the
adherent-invasive Escherichia coli from
mucus in a mucosa-comprising gut model.
NPJ Biofilms Microbiomes 2016;27(2):16016.
15. Hills RD Jr, Pontefract BA, Mishcon HR, et
al. Gut microbiome: Profound implications
for diet and disease. Nutrients 2019;11(7):
E1613.
16. Modrackova N, Bunesova V, Vlkova E, et
al. Enteral nutrition as a growth medium
for cultivable commensal bacteria and its
effect on their quantity in the stool of
children with Crohn’s disease. J Med Food
2019;22(8):810-6.
17. Nath A, Haktanirlar G, Varga Á, et al.
Biological activities of lactose-derived
prebioltics and symbiotic with probiotics
on gastrointestinal system. Medicina
(Kaunas) 2018;54(2):18.
18. Kho ZY, Lal SK. The human gut
microbiome – A potential controller of
wellness and disease. Front Microbiol
2018;9:1835.
19. Nealon NJ, Yuan L, Yang X, et al. Rice bran
and probiotics alter the porcine large
intestine and serum metabolomes for
protection against human rotavirus
diarrhea. Front Microbiol 2017;8:653.
20. Vitetta L, Vitetta G, Hall S. Immunological
tolerance and function: Associations
between intestinal bacteria, probiotics,
prebiotics, and phages. Front Immunol
2018;9:2240.
21. Azagra-Boronat I, Massot-Cladera M,
Knipping K, et al. Supplementation with
2’-FL and scGOS/FOS ameliorates
rotavirus-induced diarrhea in suckling rats.
Front Cell Infect Microbiol 2018;8:372.
22. Lee HC, Jenner AM, Low CS and Lee YK.
Effect of tea phenolics and their aromatic
fecal bacterial metabolites on intestinal
microbiota. Res Microbiol 2006;157(9):
876-84.
23. Tran THT, Everaert N, Bindelle J. Review
on the effects of potential prebiotics on
controlling intestinal enteropathogens
Salmonella and Escherichia coli in pig
production. J Anim Physiol Anim Nutr (Berl)
2018;102(1):17-32.
24. Tanabe K, Nakamura S, MoriyamaH a s h i g u c h i M , e t a l . D i e t a r y
fructooligosaccharide and glucomannan
alter gut microbiota and improve bone
metabolism in senescence-accelerated
mouse. J Agric Food Chem 2019;67(3):
867-74.
25. Li L, Xiong Q, Zhao J, et al. Inulin-type
fructan intervention restricts the increase
in gut microbiome-generated indole in
patients with peritoneal dialysis: a
randomized crossover study. Am J Clin
Nutr 2020:nqz337.
26. Sukhumsirichat W, Deesukon W, Kawakami
T, et al. Expression and characterization
of recombinant GH11 xylanase from
thermotolerant Streptomyces sp. SWU10.
Appl Biochem Biotechnol 2014;172:
436-46.
27. Hatfield RD, Rancour DM, Marita JM. Grass
cell walls: A story of cross-linking. Front
Plant Sci 2016;7:2056.
28. Florina L, Vodnar DC. Thermal processing
for the release of phenolic compounds
from wheat and oat bran. Biomolecules
2020;10(1):21.
29. Markoviak P, Slizewska K. Effect of
probiotics, prebiotics, and synbiotics on
human health. Nutrients 2017;9(9):21.
30. Chapla D, Pandit P, Shah A. Production of
xylooligosaccharides from corncob xylan
by fungal xylanase and their utilization
by probiotics. Bioresour Technol 2012;115:
215-21.
31. Monteagudo-Mera A, Rastall RA, Gibson
GR, et al. Adhesion mechanisms mediated
by probiotics and prebiotics and their
potential impact on human health. Appl
Microbiol Biotechnol 2019;103(16):6463-72.
32. Mora JA, Montero-Zamora J, Barboza N,
et al. Multi-Producct lactic acid bactria
fermentations: A Review. Fermentation
2020;6(23):6010023.
33. Monteiro CRAV, do Carmo MS, Melo BO,
et al. In vitro antimicrobial activity and
probiotic potential of Bifidobacterium and
Lactobacillus against species of
Clostridium. Nutrients 2019;11(2):448.
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Published
2020-08-31
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Woranuch K, Sakamoto T, Charoenrat T, Sukhumsirichart W. Prebiotic and antioxidant potential of feruloyl-polysaccharide from rice straw digested by recombinant xylanase enzyme of Streptomyces sp. SWU10. J Med Health Sci [Internet]. 2020 Aug. 31 [cited 2024 Dec. 25];27(2):113-24. Available from: https://he01.tci-thaijo.org/index.php/jmhs/article/view/244781
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