Diarylheptanoid 7-(3,4-Dihydroxyphenyl)-5- Hydroxy-1-Phenyl- (1E)-1-Heptene from Curcuma Comosa Roxb. Inhibits Nucleophosmin Localization and Induces Apoptosis in KG-1a Leukemic Stem Cells

Authors

  • Natsima Viriyaadhammaa Department of Medical Technology, Faculty of Associated Medical Sciences, Chiang Mai University, Mueang, Chiang Mai 50200, Thailand.
  • Suwit Duangmano Department of Medical Technology, Faculty of Associated Medical Sciences, Chiang Mai University, Mueang, Chiang Mai 50200, Thailand.
  • Pawaret Panyajai Department of Medical Technology, Faculty of Associated Medical Sciences, Chiang Mai University, Mueang, Chiang Mai 50200, Thailand.
  • Khin Khin Gyi Department of Medical Technology, Faculty of Associated Medical Sciences, Chiang Mai University, Mueang, Chiang Mai 50200, Thailand.
  • Singkome Tima Department of Medical Technology, Faculty of Associated Medical Sciences, Chiang Mai University, Mueang, Chiang Mai 50200, Thailand. Cancer Research Unit of Associated Medical Sciences (AMS CRU), Faculty of Associated Medical Sciences, Chiang Mai University, Mueang, Chiang Mai 50200, Thailand. Center of Excellence in Pharmaceutical Nanotechnology, Chiang Mai University, Mueang, Chiang Mai 50200, Thailand.
  • Sawitree Chiampanichayakul Department of Medical Technology, Faculty of Associated Medical Sciences, Chiang Mai University, Mueang, Chiang Mai 50200, Thailand. Cancer Research Unit of Associated Medical Sciences (AMS CRU), Faculty of Associated Medical Sciences, Chiang Mai University, Mueang, Chiang Mai 50200, Thailand. Center of Excellence in Pharmaceutical Nanotechnology, Chiang Mai University, Mueang, Chiang Mai 50200, Thailand.
  • Siriporn Okonogi Center of Excellence in Pharmaceutical Nanotechnology, Chiang Mai University, Mueang, Chiang Mai 50200, Thailand. Department of Pharmaceutical Sciences, Faculty of Pharmacy, Chiang Mai University, Mueang, Chiang Mai 50200, Thailand.
  • Pornngarm Dejkriengkraikul Department of Biochemistry, Faculty of Medicine, Chiang Mai University, Mueang, Chiang Mai 50200, Thailand.
  • Jeffrey Krise Pharmaceutical Chemistry, School of Pharmacy, University of Kansas, Lawrence, Kansas 66045, USA.
  • Aroonchai Saiai Department of Chemistry, Faculty of Science, Chiang Mai University, Mueang, Chiang Mai 50200, Thailand.
  • Songyot Anuchapreeda Department of Medical Technology, Faculty of Associated Medical Sciences, Chiang Mai University, Mueang, Chiang Mai 50200, Thailand. Cancer Research Unit of Associated Medical Sciences (AMS CRU), Faculty of Associated Medical Sciences, Chiang Mai University, Mueang, Chiang Mai 50200, Thailand. Center of Excellence in Pharmaceutical Nanotechnology, Chiang Mai University, Mueang, Chiang Mai 50200, Thailand.

DOI:

https://doi.org/10.31584/jhsmr.20241034

Keywords:

apoptosis, Curcuma comosa, cytotoxicity, diarylheptanoids, nucleophosmin, Zingiberaceae

Abstract

Objective: This study aimed to investigate the effect of the diarylheptanoid 7-(3,4-dihydroxyphenyl)-5- hydroxy-1-phenyl-(1E)-1-heptene (DDHPH) from the rhizome of Curcuma comosa Roxb. on nucleophosmin (NPM) protein localization followed by induced cell death in KG-1a leukemic stem cells.
Material and Methods: DDHPH was purified by column chromatography and characterized by nuclear magnetic resonance (NMR). Localization of NPM was determined by a confocal microscopy. Induction of cell death was determined by flow cytometry and Western blotting.
Results: A previous study showed that NPM directly interacted with p53 and activates p53 function. In this study, DDHPH was found to modify the translocation of NPM and enhance both p53 and cleaved caspase-3 expression. These proteins were found to control cell cycle progression and cell apoptosis induction.
Conclusion: This study demonstrated that DDHPH is a novel compound which induces cell death through NPM localization.

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Published

2024-06-21

How to Cite

1.
Viriyaadhammaa N, Duangmano S, Panyajai P, Gyi KK, Tima S, Chiampanichayakul S, Okonogi S, Dejkriengkraikul P, Krise J, Saiai A, Anuchapreeda S. Diarylheptanoid 7-(3,4-Dihydroxyphenyl)-5- Hydroxy-1-Phenyl- (1E)-1-Heptene from Curcuma Comosa Roxb. Inhibits Nucleophosmin Localization and Induces Apoptosis in KG-1a Leukemic Stem Cells. J Health Sci Med Res [Internet]. 2024 Jun. 21 [cited 2024 Jul. 17];42(4):e20241034. Available from: https://he01.tci-thaijo.org/index.php/jhsmr/article/view/271693

Issue

Vol. 42 No. 4 (2024): Jul-Aug

Section

Original Article

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