Electrospinning of Polycaprolactone Nanofibrous Scaffolds Containing Folic Acid for Nerve Tissue Engineering
DOI:
https://doi.org/10.31584/jhsmr.20231007Keywords:
electrospinning, folic acid, nerve tissue engineering, polycaprolactoneAbstract
Objective: The aim of the study was to employ electrospinning technology to fabricate aligned nanofibrous scaffolds of polycaprolactone (PCL) containing folic acid (FA) for nerve tissue engineering.
Material and Methods: Scanning electron microscopy (SEM) was used to assess the diameter distribution and degree of alignment of the nanofibers. Fourier transform infrared spectroscopy (FTIR) and powder X-ray diffraction (PXRD) were used to analyze the chemical and crystalline structures of the scaffold. Additionally, the content and release behavior of FA in the PCL fibrous scaffolds were examined. Finally, the biocompatibility of the scaffolds was evaluated using rat Schwann cells, assessing cell proliferation, alignment, and morphology.
Results: The study revealed that the nanofiber diameters ranged from 210.07 to 227.36 nm, and the scaffolds maintained an amorphous form with no effects on their chemical structure following the electrospinning process. The investigation demonstrated that PCL fibers could accommodate FA loading within a range of 99.25-102.49% w/w and that the release profile of FA followed Higuchi model. Moreover, the FA-containing PCL nanofibrous scaffolds significantly enhanced rat Schwann cell proliferation during the initial two days of culture when compared to a normal PCL nanofiber scaffold. The hydrophilic properties of folic acid are thought to have facilitated directional growth along the electrospun nanofibers, contributing to the observed results.
Conclusion: Finally, PCL-containing FA nanofibrous scaffolds may be applicable to nerve tissue engineering.
References
Daly W, Yao L, Zeugolis D, Windebank A, Pandit A. A biomaterials approach to peripheral nerve regeneration: bridging the peripheral nerve gap and enhancing functional recovery. J R Soc Interface 2012;9:202-21.
Kretschmer T, Antoniadis G, Braun V, Rath SA, Richter H-P. Evaluation of iatrogenic lesions in 722 surgically treated cases of peripheral nerve trauma. J Neurosurg 2001;94:905-12.
Chiono V, Tonda-Turo C. Trends in the design of nerve guidance channels in peripheral nerve tissue engineering. Prog Neurobiol 2015;131:87-104.
Xia H, Chen Q, Fang Y, Liu D, Zhong D, Wu H, et al. Directed neurite growth of rat dorsal root ganglion neurons and increased colocalization with Schwann cells on aligned poly(methyl methacrylate) electrospun nanofibers. Brain Res 2014;1565:18-27.
Sun B, Zhou Z, Wu T, Chen W, Li D, Zheng H, et al. Development of nanofiber sponges-containing nerve guidance conduit for peripheral nerve regeneration in vivo. ACS Appl Mater Interfaces 2017;9:26684-96.
Parker BJ, Rhodes DI, O’Brien CM, Rodda AE, Cameron NR. Nerve guidance conduit development for primary treatment of peripheral nerve transection injuries: A commercial perspective. Acta Biomater 2021;135:64-86.
Huang ZM, Zhang Y-Z, Kotaki M, Ramakrishna S. A review on polymer nanofibers by electrospinning and their applications in nanocomposites. Compos Sci Technol 2003;63:2223–53.
Sill TJ, von Recum HA. Electrospinning: applications in drug delivery and tissue engineering. Biomaterials 2008;29:1989-2006.
Zhang Y, Sun T, Jiang C. Biomacromolecules as carriers in drug delivery and tissue engineering. Acta Pharmaceutica Sinica B 2018;8:34-50.
Li L, Hao R, Qin J, Song J, Chen X, Rao F, et al. Electrospun fibers control drug delivery for tissue regeneration and cancer therapy. Adv Fiber Mater 2022;4:1375-413.
Behtaj S, Ekberg JAK, St John JA. Advances in electrospun nerve guidance conduits for engineering neural regeneration. Pharmaceutics 2022;14:219.
Tian L, Prabhakaran MP, Ramakrishna S. Strategies for regeneration of components of nervous system: scaffolds, cells and biomolecules. Regen Biomater 2015;2:31-45.
Lee JY, Bashur CA, Goldstein AS, Schmidt CE. Polypyrrolecoated electrospun PLGA nanofibers for neural tissue applications. Biomaterials 2009;30:4325-35.
Hou Y, Wang X, Zhang Z, Luo J, Cai Z, Wang Y, et al. Repairing transected peripheral nerve using a biomimetic nerve guidance conduit containing intraluminal sponge fillers. Adv Healthc Mater 2019;8:e1900913.
Cooper A, Bhattarai N, Zhang M. Fabrication and cellular compatibility of aligned chitosan–PCL fibers for nerve tissue regeneration. Carbohydr Polym 2011;85:149-56.
Ren K, Wang Y, Sun T, Yue W, Zhang H. Electrospun PCL/gelatin composite nanofiber structures for effective guided bone regeneration membranes. Mater Sci Eng C Mater Biol Appl 2017;78:324-32.
Mochane MJ, Motsoeneng TS, Sadiku ER, Mokhena TC, Sefadi JS. Morphology and properties of electrospun PCL and its composites for medical applications: a mini review. Appl Sci 2019;9.
Balashova OA, Visina O, Borodinsky LN. Folate action in nervous system development and disease. Dev Neurobiol 2018;78:391-402.
Xiao J, Zhu Y, Huddleston S, Li P, Xiao B, Farha OK, et al. Copper metal-organic framework nanoparticles stabilized with folic acid improve wound healing in diabetes. ACS Nano 2018;12:1023-32.
Harma A, Sahin MS, Zorludemir S. Effects of intraperitoneally administered folic acid on the healing of repaired tibial nerves in rats. J Reconstr Microsurg 2015;31:191-7.
Fernandez-Villa D, Jimenez Gomez-Lavin M, Abradelo C, San Roman J, Rojo L. Tissue engineering therapies based on folic acid and other vitamin b derivatives. Functional mechanisms and current applications in regenerative medicine. Int J Mol Sci 2018;19:4068.
Modupe O, Maurras JB, Diosady LL. A spectrophotometric method for determining the amount of folic acid in fortified salt. J Agric Food Res 2020;2:100060.
Parin FN, Ullah S, Yildirim K, Hashmi M, Kim IS. Fabrication and characterization of electrospun folic acid/hybrid fibers: in vitro controlled release study and cytocompatibility assays. Polymers (Basel) 2021;13:3594
Charernsriwilaiwat N, Rojanarata T, Ngawhirunpat T, Opanasopit P. Aligned electrospun polyvinyl pyrrolidone/poly ε-caprolactone blend nanofiber mats for tissue engineering. Int J Nanosci 2016;15:1650005.
Zhang L, Webster TJ. Nanotechnology and nanomaterials: Promises for improved tissue regeneration. Nano Today 2009;4:66-80.
Parın FN, Aydemir Çİ, Taner G, Yıldırım K. Co-electrospunelectrosprayed PVA/folic acid nanofibers for transdermal drug delivery: Preparation, characterization, and in vitro cytocompatibility. J Ind Text 2021;51:1323S-47.
Yang H, Wang N, Yang R, Zhang L, Jiang X. Folic aciddecorated beta-cyclodextrin-based poly(epsilon-caprolactone)-dextran star polymer with disulfide bond-linker as theranostic nanoparticle for tumor-targeted mri and chemotherapy. Pharmaceutics 2021;14:52.
Akhgari A, Iraji P, Rahiman N, Hasanzade Farouji A, Abbaspour M. Preparation of stable enteric folic acid-loaded microfiber using the electrospinning method. Iran J Basic Med Sci 2022;25:405-13.
Karuppuswamy P, Reddy Venugopal J, Navaneethan B, Luwang Laiva A, Ramakrishna S. Polycaprolactone nanofibers for the controlled release of tetracycline hydrochloride. Mater Lett 2015;141:180-6.
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