Phytochemical Analysis and Evaluation of Antioxidant and Antibacterial Activities of Kratom-Containing Formulations of Ya-Gae-Bid-Na-Ron
DOI:
https://doi.org/10.31584/jhsmr.20231006Keywords:
antibacterial, antioxidant, kratom, Ya-Gae-Bid-Na-RonAbstract
Objective: This study aimed to evaluate the antioxidant and antibacterial activities of Ya-Gae-Bid-Na-Ron (YGBNR).
Material and Methods: YGBNR formulations were prepared using kratom collected from different provinces in Thailand. Mitragynine content was determined using high-performance liquid chromatography (HPLC), while total phenolic content (TPC), total tannin content (TTC), and total flavonoid content (TFC) were determined using colorimetric methods. Antioxidant power was estimated using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) and ferric-reducing antioxidant power (FRAP) assays. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of YGBNR were determined using a broth dilution technique and plating of the bacterial solution, respectively.
Results: Mitragynine content, TPC, TTC, and TFC varied among different sources of kratom. DPPH assay indicated that YGBNR had an average IC50 of 0.44±0.04 mg/mL, and the FRAP assay ranged from 61.48±0.55 to 102.98±0.94 mg gallic acid equivalent (GAE), per gram of dry extract. YGBNR extracts did not exhibit any antibacterial activity. However, the volatile oil fraction showed MIC against Escherichia coli and Pseudomonas aeruginosa at a concentration of 3 mg/mL.
Conclusion: Phytochemical analysis revealed the abundance of phenolic compounds in YGBNR. YGBNR exhibits promising antioxidant potential, but does not exert any antibacterial activity.
References
World Health Organization. Kratom (Mitragyna speciosa), mitragynine, and 7-hydroxymitragynine, 11-15 Oct 2021 [homepage on the Internet]. Geneva: WHO; 2021 [cited 2023 Jul 18]. Availale from: https://cdn.who.int/
Takayama H. Chemistry and pharmacology of analgesic indole alkaloids from the Rubiaceous plant, Mitragyna speciosa. Chem Pharm Bull 2004;52:916-28.
Matsumoto K, Mizowski M, Suchitra T, Takayama H, Sakai SI, Aimi N, et al. Antinociceptive action of mitragynine in mice: evidence for the involvement of supraspinal opioid receptors. Life Sci 1996;59:1149-455.
Thongpraditchote S, Matsumoto K, Tohda M, Takayama H, Aimi N, Sakai SI, et al. Identification of opioid receptor subtypes in antinociceptive actions of supraspinally-administered mitragynine in mice. Life Sci 1998;62:1371-8.
Kruegel AC, Gassaway MM, Kapoor A, Varadi A, Majumdar S, Filizola M, et al. Synthetic and receptor signaling explorations of the Mitragyna alkaloids: mitragynine as an atypical molecular framework of opioid receptor modulators. J Am Chem Soc 2016;138:6754-64.
Tsuchiya S, Miyashita S, Yamamoto M, Horie S, Sakai SI, Aimi N, et al. Effect of mitragynine, derived from Thai folk medicine, on gastric acid secretion through opioid receptor in anesthetized rats. Eur J Pharmacol 2002;443:185-8.
Kumarnsit E, Keawpradub N, Nuankaew W. Acute and longterm effects of alkaloid extract of Mitragyna speciosa on food and water intake and body weight in rats. Fitoterapia 2006;77:339-45.
Chittrakarn S, Sawangjaroen K, Prasettho S, Janchawee B, Keawpradub N. Inhibitory effects of kratom leaf extract (Mitragyna speciosa Korth.) on the rat gastrointestinal tract. J Ethnopharmacol 2008;116:173-8.
Limsuwanchote S, Putalun W, Keawpradub N, Tanaka H, Morimoto S, Wungsintaweekul J. Anti-mitragynine monoclonal antibody-based ELISA for determination of alkaloids in the kratom cocktail. Forensic Toxicol 2017;35:167-72.
Singleton VL, Orthofer R, Lamuela-Raventós RM. Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin-Ciocalteu reagent. Meth Enzymol 1999;299:152-78.
Rebaya A, Belghith SI, Baghdikian B, Leddet VM, Mabrouki F, Olivier E, et al. Total phenolic, total flavonoid, tannin content, and antioxidant capacity of Halimium halimifolium (Cistaceae). J Appl Pharm Sci 2014;5:52-7.
Santiago-Adame R, Medina-Torres L, Gallegos-Infante JA, Calderas F, Gonzãlez-Laredo RF, Rocha-Guzmán NE, et al. Spray drying-microencapsulation of cinnamon infusions (Cinnamomum zeylanicum) with maltodextrin. LWT-Food Sci Technol 2015;64:571-7.
Seiquer I, Rueda A, Olalla M, Cabrera-Vique C. Assessing the bioavailability of polyphenols and antioxidant properties of extra virgin argan oil by simulated digestion and Caco-2 cell assays, Comparative study with extra virgin olive oil. Food Chem 2015;188:496–503.
Benzie IFF, Strain JJ. Ferric reducing/antioxidant power assay: direct measure of total antioxidant activity of biological fluids and modified version for simultaneous measurement of total antioxidant power and ascorbic acid concentration. Meth Enzymol 1999;299:15-27.
Wungsintaweekul B. Antibacterial and antioxidant activities of methanolic leaf extracts of some Annonaceous plants found in Nakhon Si Thammarat, Thailand. J Appl Pharm Sci 2023;13:84-95.
Nakaphan T, Teerachaisakul M, Puttum S, Sompimai K, Nootim P. Traditional use of kratom (Mitragyna speciosa Korth.) among folk healers in Southern Thailand. J Thai Trad Alt Med 2016;14:274-84.
Limcharoen T, Pouyfung P, Ngamdokmai N, Prasopthum A, Ahmad AR, Wisdawati W, et al. Inhibition of α-glucosidase and pancreatic lipase properties of Mitragyna speciosa (Korth.) Havil. (Kratom) leaves. Nutrients 2022;14:3909.
Parthasarathy S, Bin Azizi J, Ramanathan S, Ismail S, Sasidharan S, Said MI, et al. Evaluation of antioxidant and antibacterial activities of aqueous, methanolic and alkaloid extracts from Mitragyna speciosa (Rubiaceae Family) leaves. Molecules 2009;14:3964-74.
Sengnon N, Vonghirundecha P, Chaichan W, Juengwatanatrakul T, Onthong J, Kitprasong P, et al. Seasonal and geographic variation in alkaloid content of kratom (Mitragyna speciosa (Korth.) Havil.) from Thailand. Plants 2023;12:949.
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