Effects of Trehalose and Sucrose on Human Sperm Motility, Vitality and Morphology after Cryopreservation

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Manaphat Suksai
Kriengsak Dhanaworavibul


Objective: To compare human sperm motility, vitality and morphology after cryopreservation among sucrose, and different trehalose concentrations.
Material and Methods: A total number of 124 normozoospermic semen samples were collected. Each semen sample was divided into 4 portions, and cryopreserved in a human sperm-preserving medium along with cryoprotectants, including; 50 milimolar (mM) trehalose, 100 mM trehalose, 200 mM trehalose and 50 mM sucrose, respectively. All semen samples were frozen by using a vapor phase method. Post-thawed sperm motility, vitality and morphology were assessed. R program was used for data analysis. A p-value of <0.05 was considered statistically significant.
Results: Post-thawed semen evaluation indicated that 50 mM trehalose was better than 50 mM sucrose in all sperm parameters, which included progressive motility (p-value=0.037, total motility (p-value<0.001), vitality (p-value<0.001) and morphology (p-value<0.001). The sperm parameters were not significantly different among 100 mM trehalose, 200 mM trehalose and 50 mM sucrose.
Conclusion: The use of 50 mM trehalose, as non-permeating cryoprotectant, showed superior post-thaw sperm
parameters over sucrose, and other trehalose concentrations.


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Suksai M, Dhanaworavibul K. Effects of Trehalose and Sucrose on Human Sperm Motility, Vitality and Morphology after Cryopreservation. J Health Sci Med Res [Internet]. 2019Mar.18 [cited 2020Jul.8];37(2):101-7. Available from: https://he01.tci-thaijo.org/index.php/jhsmr/article/view/148584
Original Article


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