Exploration of microorganisms in hospital operating room, university classroom and meeting room by modified sampling collector compared to open dish method

Abstract

Six locations aroud the room and four corners of operating bed in hospital operating room were investigated for microorganisms using modified sample collector. The result showed that at least 8 genus of fungi (Aspergillus sp., Cladosporium sp., Mucor sp., Rhizopus sp., Penicillium sp., Scopulariopsis sp., non sporulating fungi, Yeasts) and 6 genus of bacteria (Staph. saprophyticus, Staph. epidermidis, Micrococcus sp., Bacillus sp., Flavobacterium sp. and Diphtheroid sp.) were found .The floor cleaning after operation with anti-infectious agent mopping could not decrease the total number of fungi (333/301, 139/172) or bacteria (372/471, 196/254) both in locations around the room and the corners of operation bed. The same results were found in university classroom with at least 7 genus of fungi (Aspergillus niger., Aspergillus sp., Cladosporium sp., Rhizopus sp., Penicillium sp., Fusarium sp., non sporulating fungi) and 3 genus of bacteria (Staph.coagulase neg., Bacillus sp., Acinetobacter lwoffii) were found and after mopping, the total number of fungi (333/301, 139/172) and bacteria (372/471, 196/254) did not decrease. After changing the cleaning method by using anti-dust agent mopping floor of classroom, the result showed 5 times decreasing in the number of fungi (8/43). In addition, comparing to open disk method, the modified sample collector could collect more genus and more number in every exporation. Bull Chiang Mai Assoc Sci 2009; 42: 25-36.