Detection of Mia genotype of DNA extracted from blood and hair roots by Polymerase Chain Reaction

Abstract

Objective: To detect Mia genotype of DNA extracted from blood and hair roots by Polymerase Chain Reaction (PCR) method.

Method: One hundred and forty two blood group O and B samples were obtained from Medical Technology’s students, Chiang Mai University. Phenotyping was performed by serological method. Genomic DNA of blood and hair roots samples were extracted from 59 samples including 29 Mi(a+) and 30 Mi(a-) random samples. Genotype testing was performed by PCR method.

Results: The phenotyping of 142 samples showed that 29 samples were Mi(a+) and 113 samples were Mi(a-). The genotype testing of 59 DNA samples, which extracted from blood and hair roots samples of the individual subject, were concordant. The comparison results between PCR and serology methods from 59 samples were also in agreement.

Conclusion: The PCR method can be used to determine Mia genotype in blood and hair root samples. The advantages of detection of Mia genotype by PCR technique were helpful to select compatible blood for

recipients with anti-Mia to prevent adverse effects of transfusion and investigate causes of hemolytic disease of the newborn. Bull Chiang Mai Assoc Med Sci 2009; 42: 242-250.