Detection of weak A antigen associated with Proteus vulgaris infection
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Abstract
Background: A person with weak A antigen expression is presented.
Objectives: The study aimed to reveal weak A antigen by different methods applied in laboratories.
Materials and methods: The reaction of adsorption, agglutination, and inhibition of agglutination were performed at 4 °C, 10 °C, 13 °C, 20 °C, and 37 °C for 1-13 hrs and one-day incubation. The complement of the guinea pig was added to the heated serum to reveal hemolysis.
Results: A person’s red blood cells reacted with commercial anti-B sera. Whereas anti-A and anti-B (reactive at 37 °C) antibodies were found in the serum. The person demonstrated anti-A antibody adsorbing ability at 4 °C. The plasma of the person inhibited agglutination of A and B RBCs by polyclonal sera. The blood culture was positive for Proteus vulgaris infection.
Conclusion: The presence of weak A antigen was detected the best by the adsorption method at low temperatures with polyclonal sera from the O blood group.
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Personal views expressed by the contributors in their articles are not necessarily those of the Journal of Associated Medical Sciences, Faculty of Associated Medical Sciences, Chiang Mai University.
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