Effects of Low-Dose X-ray on Oxidative State, Lipid Peroxidation, and Membrane Fluidity of Human Peripheral Blood Mononucleated Cells
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Abstract
Background: There is a concern about the effects of low-dose radiation used in medical applications due to risk of biological effects, as these effects have not been fully evaluated.
Objectives: This study aimed to evaluate the effects of low-dose X-ray on the intracellular reactive oxygen species (ROS), lipid peroxidation, membrane fluidity, and cell viability of human peripheral blood mononuclear cells (PBMCs).
Materials and methods: Cells were irradiated using an X-ray generator at the radiation energy of 120 kVp to obtain the absorption dose of 0.05, 0.1 and 0.2 Gy. Fluorescent probe 2′7′-dichlorofluorescein diacetate (DCFH-DA) was used to evaluate intracellular reactive oxygen species of PBMCs. Thiobarbituric acid reactive substances assay (TBARS) was applied to determine malondialdehyde (MDA) level which is an indicative of lipid peroxidation. Membrane fluidity was also determined by fluorescence anisotropy of the fluorescent probe 1,6-diphenyl-1,3,5-hexatriene (DPH). Finally, cell viability was determined by resazurin assay.
Results: The instant effects of low-dose X-rays show a significant decrease in ROS level at 0.1 and 0.2 Gy. MDA level per cell of non-irradiated PBMCs was 6.12±1.67 (SD) fmole per cell. There are no significant alteration of MDA level and membrane fluidity from the effects of X-ray at doses up to 0.2 Gy. The cell viability at 72 hours after irradiation at 0.2 Gy shows a significant decreased.
Conclusion: Low-dose of X-rays on human peripheral blood mononuclear cells (PBMCs) shows a significant decrease in the intracellular reactive oxygen species (ROS) (0.1 and 0.2 Gy) and cell dead (0.2 Gy).
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Personal views expressed by the contributors in their articles are not necessarily those of the Journal of Associated Medical Sciences, Faculty of Associated Medical Sciences, Chiang Mai University.
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