Bacterial contamination and their tolerance in banknotes and coins surrounding the area of Chiang Mai University Hospital in Chiang Mai Province

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Ponrut Phunpae
Chanjuti Sriruan
Ratchadaporn Udpoun
Santhana Buamongkol
Sudjai Pawichai
Autchar Ruangpayuk
Watchawan Chairuangwut

Abstract

Background: Currency including banknotes and coins are used in everyday life by everyone. It is, therefore, a universal medium for carrying potentially pathogenic bacteria which can spread and cause infection in the population. Thus, it is of interest to study the bacterial contamination in currency used daily in Chiang Mai province, of which there is scant information.


Objectives: To study the quantity and types of bacterial contamination on banknotes and coins circulating in the area surrounding Chiang Mai University Hospital in Chiang Mai province and examine the tolerance of detected bacteria on the currency.


Materials and methods: Banknotes and coins, totaling 343 samples, were collected from circulation in the area surrounding Chiang Mai University Hospital in Chiang Mai. The bacterial contamination was studied based on culture methods and identified by morphological and biochemical tests. To examine the bacterial tolerance, the contaminated bacteria recovered from the currency, including Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa and Acinetobacter baumannii, were tested. These bacteria were inoculated on banknotes and coins and the viability of the bacteria at various time points was examined.


Results: The amount of bacterial contamination on banknotes, which are linen fibers, was higher than those on coins, which are opaque metals. The gram-positive bacteria were found in a higher amount than gram-negative bacteria, in both banknotes and coins. Human skin flora and pathogenic bacteria, especially S. aureus and pathogenic Enterobacteriaceae, were detected. The bacterial tolerance study revealed that S. aureus, the gram-positive cocci, showed a decrease of viability on banknotes and coins within 6 hours. Meanwhile, gram-negative bacilli presented a decrease of viability in 6 and 1 hours on banknotes and coins, respectively. The bacterial tolerance testing by used and unused currency showed the same results.


Conclusion: All banknotes and coins circulating in the area surrounding Chiang Mai University Hospital in Chiang Mai province were demonstrated to be contaminated with bacteria, some of which might be pathogens and dangerous to users. Gram-positive bacteria has higher tolerance than gram-negative bacteria on currency. Bacterial tolerance on banknotes is longer than on coins. The knowledge obtained is advantageous for people in general as an awareness of these harmful bacteria when using the currency.

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How to Cite
Phunpae, P., Sriruan, C., Udpoun, R., Buamongkol, S., Pawichai, S., Ruangpayuk, A., & Chairuangwut, W. (2018). Bacterial contamination and their tolerance in banknotes and coins surrounding the area of Chiang Mai University Hospital in Chiang Mai Province. Journal of Associated Medical Sciences, 51(3), 171-179. Retrieved from https://he01.tci-thaijo.org/index.php/bulletinAMS/article/view/136262
Section
Medical Technology

References

[1] El-Dars FM, Hassan WM. A preliminary bacterial study of Egyptian paper money. Int J Environ Health Res. 2005; 15(3): 235-9.

[2] Basavarajappa KG, Rao PN, Suresh K. Study of bacterial, fungal, and parasitic contamination of currency notes in circulation. Indian J Pathol Microbiol. 2005; 48(2): 278-9.

[3] Xu J, Moore JE, Millar BC. Ribosomal DNA (rDNA) identification of the culturable bacterial flora on monetary coinage from 17 currencies. J Environ Health. 2005; 67(7): 51-5.

[4] Khin NO, Phyu PW, Aung MH, Aye T. Contamination of currency notes with enteric bacterial pathogens. J. Diarrhoeal. Dis. Res. 1989, 7(3-4): 92-4

[5] Kuria JK, Wahome RG, Jobalamin M, Kariuki SM. Profile of bacteria and fungi on money coins. East Afr Med J. 2009; 86(4): 151-5.

[6] Yazah AJ, Yusuf J, Agbo J. Bacterial Contaminants of Nigerian Currency Notes and Associated Risk Factors. Res. J. Med. Sci.2012 ; 6(1): 1-6.

[7] Kalita M, Palusińska-Szysz M, Turska-Szewczuk A, Wdowiak-Wrobel S, Urbanik-Sypniewska T. Isolation of cultivable microorganisms from Polish notes and coins. Pol J Microbiol. 2013; 62(3): 281-6.

[8] The Treasury Department: Thai coins [Internet]. Bangkok; 2561 [cited 2018 5 May]. Available from: https://www.m-society.go.th/ewt_news.php?nid=16328.html.

[9] The Bank of Thailand: Volume and Value of Notes in Circulation [Internet]. Bangkok; 2561 [cited 2018 5 May]. Available from: https://www.bot.or.th/Thai/Banknotes/Pages/banknoteinfo.aspx.html.

[10] Hugo WB, Denyer SP, Norman AH, Gorman SP, Russel AD. Pharmaceutical Microbiology. Oxford:Blackwell Scientific; 1983.

[11] Igumbor EO, Obi CL, Bessong PO, Potgieter N, Mkasi TC. Microbiological analysis of banknotes circulating in the Venda region of Limpopo province, South Africa. S. Afr. j. sci. 2007; 103(9-10): 365-6.

[12] Vriesekoop F, Chen J, Oldaker J, Besnard F, Smith R, Leversha W, et al. Dirty Money: A Matter of Bacterial Survival, Adherence, and Toxicity. Microorganisms. 2016; 4(4): 42.

[13] Vriesekoop F, Russell C, Alvarez-Mayorga B, Aidoo K, Yuan Q, Scannell A, et al. Dirty money: an investigation into the hygiene status of some of the world's currencies as obtained from food outlets. Foodborne Pathog Dis. 2010 Dec;7(12):1497-502.

[14] Mackintosh CA, Hoffman PN. An extended model for transfer of micro-organisms via the hands: differences between organisms and the effect of alcohol disinfection. J. Hyg. 1984; 92(3): 345-55.

[15] Sawalha H, Mowais MA. Bacterial Contamination of Paper Banknotes in Circulation; a Case Study in the Jenin District, Palestine. Journal of Science. 2012; 1(2): 36-9.

[16] de Carvalho CC, Caramujo MJ. Bacterial diversity assessed by cultivation-based techniques shows predominance of Staphylococccus species on coins collected in Lisbon and Casablanca. FEMS Microbiol Ecol. 2014; 88(1):26-37.

[17] Mehrad B, Clark N, Zhanel G, Lynch J. Antimicrobial Resistance in Hospital-Acquired gram-negative Bacterial Infections. CHEST 2015; 147(5): 1413-21.

[18] Prasai T, Yami KD, Joshi DR. Microbial load on paper/polymer currency and coins. Nepal Journal of Science and Technology. 2008; 9: 105-9.

[19] Lopez GU, Gerba CP, Tamimi AH, Kitajima M, Maxwell SL, Rose JB. Transfer efficiency of bacteria and viruses from porous and nonporous fomites to fingers under different relative humidity conditions. Appl Environ Microbiol. 2013 Sep;79(18):5728-34.

[20] Santo CE, Quaranta D, Grass G. Antimicrobial metallic copper surfaces kill Staphylococcus haemolyticus via membrane damage. MicrobiologyOpen. 2012 ;1(1): 46-52.

[21] Odonkor ST, Ampofo JK. Escherichia coli as an indicator of bacteriological quality of water: an overview. Microbiology Research 2013; volume 4:e2

[22] Miller LG, Eells SJ, David MZ, Taylor NOR, Kumar N, Cruz D, et al. Staphylococcus aureus Skin Infection Recurrences Among Household Members: An Examination of Host, Behavioral, and Pathogen-Level Predictors. Clin Infect Dis 2015; 60: 753–63.

[23] Conway T and Cohen PS. Commensal and Pathogenic Escherichia coli Metabolism in the Gut. Microbiol Spectr. 2015; 3(3): doi: 10.1128/microbiolspec.MBP-0006-2014.

[24] Kramer A, Schwebke I, Kampf G How long do nosocomial pathogens persist on inanimate surfaces?BMC Infectious Diseases 2006; 6: 130. doi:10.1186/1471-2334-6-130