Feasibility of high resolution melting curve analysis for rapid serotyping of Salmonella from hospitalised patients

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Published: Nov 12, 2018
Keywords:
Salmonella Serotyping HRM-PCR

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Kritchai Poonchareon
Division of Biochemistry, School of Medical Sciences, University of Phayao, Phayao Province, Thailand
Chaiwat Pulsrikarn
Department of Medical Sciences, WHO National Salmonella and Shigella Center, National Institute of Health, Ministry of Public Health, Nonthaburi Province, Thailand
Sukon Khamvichai
Phayao Ram Hospital, Phayao Province, Thailand
Pakpoom Tadee
Integrative Research Center for Veterinary Preventive Medicine, Department of Food Animal Clinics, Faculty of Veterinary Medicine, Chiang Mai University, Chiang Mai Province, Thailand

Abstract

Background: Serum agglutination test is the gold standard phenotyping method widely used for Salmonella enterica characterisation.This conventional method is limited by its complicated and time-consuming procedures. High resolution melting curve (HRM) analysis is introduced as a rapid and labour-saving method.


Objectives: To compare the results of conventional serum agglutination and quantitative PCR-HRM analysis to assess the feasibility of this alternative approach for Salmonella serotyping.


Materials and methods: Salmonella strains from 38 human-originating samples were serotyped using the conventional serum agglutination method and HRM analysis.


Results: The conventional serum agglutination assay detected 14 serotypes, while the HRM analysis identified 10 HRM profiles. There was a correlation between most of the serotyping results obtained by the two methods. Nine of the HRM profiles were unique to a single serotype, of each. One exception was HRM_3. Many of the indistinct curves that were grouped in this HRM pattern belonged to five Salmonella serotypes, including Weltevreden, Corvallis, Derby, Kedougou and Kentucky.


Conclusion: It is difficult to determine all Salmonella serotypes by HRM analysis. However, this method can be used as an alternative to the conventional serum agglutination assay for rapid and labour-saving serotyping.

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How to Cite
Poonchareon, K., Pulsrikarn, C., Khamvichai, S., & Tadee, P. (2018). Feasibility of high resolution melting curve analysis for rapid serotyping of Salmonella from hospitalised patients. Journal of Associated Medical Sciences, 52(1), 37–41. Retrieved from https://he01.tci-thaijo.org/index.php/bulletinAMS/article/view/134519
Issue
Vol. 52 No. 1 (2019): January-April
Section
Research Articles
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Personal views expressed by the contributors in their articles are not necessarily those of the Journal of Associated Medical Sciences, Faculty of Associated Medical Sciences, Chiang Mai University.

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