Antigen detection of Fasciola gigantica eggs using Indirect ELISA and Western blot techniques
Keywords:
Fasciola gigantica, Hatching, Egg secreted proteinAbstract
Fasciolosis are foodborne zoonotic trematode parasites and major pathogens of cattle and sheep. Fasciolosis causes an economic loss of livestock industry, meat and milk from the cattle, and the disease in humans. The mature worm produces eggs, the egg are released with the feces in natural water resources and hatching into the parasites. Various genes and proteins are involved in different processes of parasite. The aim of this study was to detect specific protein expression for hatching of F. gigantica egg using Indirect ELISA and Western blot techniques. The results showed that Indirect ELISA could detect FgTRP14, FgSOD, FgTrx, FgCatL3, FgCatL1H, FgLAP and FgTGR proteins in Fasciola egg on days 0, 9 and 14. In addition, immunoblots of whole body of egg protein on day 0, 9 and 14 were positively reacted with rabbit anti-rFgTrx, rFgCatL1H and rFgSOD. The identification of proteins actively secreted by live F. gigantica eggs provides an important new information for understanding immune modulation and it may be a good effective target for the diagnosis development
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