ระดับ Hemeoxygenase-1 ในเลือดคนงานที่สัมผัสซิลิกาจากการทำงาน ในโรงโม่หินเขตภาคเหนือตอนบน

บทคัดย่อ

This study aimed to identify the relationship between blood Hemeoxygenase-1 (HO-1) levels and three parameters: 1) chest x-ray result, 2) pulmonary function test, 3) silica levels in workplace. The serum HOI levels, pulmonary function test and chest X-ray were performed among 299 stone mill workers in 9 stone mills. The silica dust was also measured for 3 points in each workplace during work time for 8 hours using air sampling equipment. The silica dust and serum HO-1 levels were analyzed by spectrophotometer and ELISA with sandwish enzyme immunoassay technique, respectively. All chest x-ray films were read and analyzed by specialist. The pulmonary function test parameters including FVC, FEV1 and FEV1/FVC were used. Analysis of the relationship was performed via Anova Statistics, Spearman correlation test. There were no lung lesions in of chest X-ray in stone mill workers. The silica levels in 9 stone mills were 1.30, 1.99, 6.34, 1.10, 15.08, 15.91, 6.31, 11.25 and 1.67 mg/m3 respectively of these such levels were over ACGIH standard (0.025 mg/m3). The average of HO-1 levels in serum was 320.27+207.68 ng/ml. Additionally the average of percentage of FVC and FEV1 compared with predicted value were 85.90 , 13.58 , 91.58±16.76 and the average of percentage of FEV1/FVC was 109.53+15.4 respectively. The correlation between serum HO-1 levels and silica levels was positive significance (r = 0.419, p<0.01) whereas the correlation between serum HO-1 levels and percentage of FEV1/FVC was negative significance (l- = -0.219, p<0.01). Although the correlation between serum HO-1 levels and silica levels was positive and the corre¬lation between serum HO-1 levels and percentage of FEV1/FVC was negative but the result of chest x-ray film was normal, this study showed that by determining the blood HO-1 levels, It was sensitive enough to be used as indicator for early detection of lung's pathology in the workers who were exposed to silica dust. It could be move to do in any sites and any factories and be the early screening test to silicosis prevention which was