Insecticide susceptibility of genetically pyrethroid-resistant Aedes aegypti mosquitoes

Authors

  • Jakkrawarn Chompoosri National Institute of Health, Department of Medical Sciences
  • Ratana Tacharoenmuang National Institute of Health, Department of Medical Sciences
  • Chayada Khamsawads National Institute of Health, Department of Medical Sciences
  • Nittaya Methawanitphong National Institute of Health, Department of Medical Sciences
  • Jariya Krutbut National Institute of Health, Department of Medical Sciences
  • Thanyapak Makruen National Institute of Health, Department of Medical Sciences
  • Archawin Rojanawiwat National Institute of Health, Department of Medical Sciences

DOI:

https://doi.org/10.14456/dcj.2021.112

Keywords:

insecticide susceptibility, mutation, para gene, sodium channel protein, genetically pyrethroid-resistant Ae. aegypti mosquitoes

Abstract

Genetic resistance to pyrethroids in Aedes aegypti mosquitoes causes an ineffective use of pyrethroids for the control of Ae. aegypti-borne diseases. This reasearch aimed to study the insecticide susceptibility of genetically pyrethroid-resistant Ae. aegypti mosquitoes. Ae. aegypti mosquitoes from Nakhon Ratchasima province were exposed to 10 insecticides for susceptibility status by WHO susceptibility test. Polymerase chain reaction (PCR) and DNA sequencing were conducted for detecting mutations in target site for pyrethroids in domain II of sodium channel protein on para gene in pyrethroid-resistant Ae. aegypti mosquitoes. The results showed that Ae. aegypti mosquitoes from Nakhon Ratchasima province were completely susceptible to fenitrothion and malathion in organophosphates including propoxur and bendiocarb in carbamates with mean mortality rates at 24 h of 100±0%. However, the mosquitoes were resistant to all 6 pyrethroid insecticides with the mean mortality rates varied between 9.00±3.83% and 26.00±5.16%. The mean mortality rates of mosquitoes were significantly different among insecticides (p<0.05). Based on the analysis of 60 partial nucleotide sequences of para gene, 35 sequences were positive for two point mutations by amino acid substitutions of S989P and V1016G in sodium channel protein. The mutation frequency in the analyzed sequences of the pyrethroid-resistant mosquitoes was 0.58. It suggests that organophosphates and carbamates could be alternative insecticides to pyrethroids for the control of genetically pyrethroid-resistant Ae. aegypti mosquitoes. However, those insecticides should be used sparingly and integrated with the safe mosquito control measures.

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References

Limkittikul K, Brett J, L’Azou M. Epidemiological Trends of Dengue Disease in Thailand (2000-2011): A Systematic Literature Review. PLoS Negl Trop Dis 2014;8(11):e3241.

Bureau of Vector-Borne Diseases Control, Department of Disease Control. Dengue Fever [Internet]. [cited 2021 Jan 11]. Available from: https://drive.google.com/drive/folders/1TTaSvaYYamVwA5Ig7ATZJmIcHBuGXOSb (in Thai)

World Health Organization. Zika virus [Internet]. [cited 2021 Feb 27]. Available from: https://www.who.int/news-room/fact-sheets/detail/zika-virus

Bureau of Vector-Borne Diseases Control, Department of Disease Control. Zika virus [Internet]. [cited 2021 Jan 19]. Available from: https://drive.google.com/file/d/18QD6uwaQ844gfsZSGWA01puklko1hKCM/view (in Thai)

Division of Epidemiology, Department of Disease Control (TH). Reporting of Priority Diseases Guideline, Thailand. 2012. 30 p. (in Thai)

Division of Vector borne Diseases, Department of Disease Control. Reporting of Insecticide Susceptibility in Mosquito Vectors [Internet]. [cited 2020 Dec 10]. Available from: https://drive.google.com/drive/folders/1Kzxz3iEgyAzNpIDSEWJ0QTy_eVoS7FJY (in Thai)

Chareonviriyaphap T, Bangs MJ, Suwonkerd W, Kongmee M, Corbel V, Ngoen-Klan R. Review of insecticide resistance and behavioral avoidance of vectors of human diseases in Thailand. Parasit Vectors. 2013;6:280.

World Health Organization. Safety of pyrethroids for public health use [Internet]. [cited 2020 Dec 10]. Available from:https://www.who.int/publications/i/item/WHO.PCS.RA.2005.1

Soderlund DM, Bloomquist JR. Molecular mechanisms of insecticide resistance. In: Roush RT, Tabashnikin BE, editors. Pesticide Resistance in Arthropods. New York: Chapman and Hall; 1990. p. 58-96.

Fan Y, O’Grady P, Yoshimizu M, Ponlawat A, Kaufman PE, Scott JG. Evidence for both sequential mutations and recombination in the evolution of kdr alleles in Aedes aegypti. PLoS Negl Trop Dis. 2020;14(4):e0008154.

Sirsawat R, Komalamisra N, Apiwathnasorn C, Paeporn P, Roytrakul S, Rongsriyam Y, et al. Field-collected permethrin-resistant Aedes aegypti from central Thailand contain point mutatioms in the domain IIS6 of the sodium channel gene (kdr) Southeast Asian J Trop Med Public Health. 2012;43(6):1380-6

Stenhouse SA, Plernsub S, Yanola J, Lumjuan N, Dantrakool A, Choochote W, et al. Detection of the V1016G mutation in the voltage-gated sodium channel gene of Aedes aegypti (Diptera: Culicidae) by allele-specific PCR assay, and its distribution and effect on deltamethrin resistance in Thailand. Parasit Vectors. 2013;6:253.

Yanola J, Somboon P, Walton C, Nachaiwieng W, Somwang P, Prapanthadara L. Highthroughput assays for detection of the mutation in the voltage‐gated sodium channel gene in permethrin‐resistant Aedes aegypti and the distribution of this mutation throughout Thailand. Trop Med Int Health. 2011;16(4):501-9.

Sri-ard B. Preliminary research. 3rd ed. Bangkok: Suweriyasan; 1992. (in Thai)

World Health Organization. Monitoring and managing insecticide resistance in Aedes mosquito populations. Interim guidance for entomologists [Internet]. [cited 2021 Feb 27]. Available from: https://apps.who.int/iris/bitstream/handle/10665/204588/WHO_ZIKV_VC_16.1_eng.pdf?sequence=2&isAllowed=y

Chung HH, Cheng IC, Chen YC, Lin C, Tomita T, Teng HJ. Voltage-gated sodium channel intron polymorphism and four mutations comprise six haplotypes in an Aedes aegypti population in Taiwan. PLoS Negl Trop Dis. 2019;13(3):e0007291. https://doi.org/10.1371/journal.pntd.0007291

Abbott W. A method of computing the effectiveness of an insecticide. J Econ Entomol. 1925;18:265-7.

Martin-Reina J, Duarte JA, Cerrillos L, Bautista JD, Moreno I. Insecticide Reproductive Toxicity Profile: Organophosphate, Carbamate and Pyrethroids. J Toxins. 2017;4(1):7.

Liu N. Insecticide resistance in mosquitoes: impact, mechanisms, and research directions. Annu Rev Entomol. 2015;60:537-59.

Haddi K, Tomé HVV, Du Y, Valbon WR, Nomura Y, Martins GF, et al. Detection of a new pyrethroid resistance mutation (V410L) in the sodium channel of Aedes aegypti: a potential challenge for mosquito control. Sci Rep. 2017;7:46549. doi: 10.1038/srep46549.

Du Y, Nomura Y, Satar G, Hu Z, Nauen R, He SY, et al. Molecular evidence for dual pyrethroid-receptor sites on a mosquito sodium channel. Proc Natl Acad Sci U S A. 2013;110(29):11785-90. doi: 10.1073/pnas. 1305118110.Epub 2013 Jul 2

Srisawat R, Komalamisra N, Eshita Y, ZhengM, Ono K, Itoh TQ, et al. Point mutations in domain II of the voltage-gated sodium channel gene in deltamethrin-resistant Aedes aegypti (Diptera: Culicidae). Appl Entomol Zool. 2010;45:275-82.

Yanola J, Somboon P, Walton C, Nachaiwieng W, Prapanthadara L. A novel F1552/C1552 point mutation in the Aedes aegypti voltage-gated sodium channel gene associated with permethrin resistance. Pesti Biochem Physiol. 2010;96:127-31.

Plernsub S, Saingamsook J, Yanola J, Lumjuan N, Tippawangkosol P, Walton C, et al. Temporal frequency of knockdown resistance mutations, F1534C and V1016G, in Aedes aegypti in Chiang Mai city, Thailand and the impact of the Insecticide susceptibility of Aedes aegypti mosquitoes mutations on the efficiency of thermal fogging spray with pyrethroids. Acta Trop. 2016;162:125-32.

Plernsub S, Saingamsook J, Yanola J, Lumjuan N, Tippawangkosol P, Sukontason K, et al. Additive effect of knockdown resistance mutations, S989P, V1016G and F1534C, in a heterozygous genotype conferring pyrethroid resistance in Aedes aegypti in Thailand. Parasit Vectors. 2016;9:417.

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Published

2021-12-29

How to Cite

1.
Chompoosri J, Tacharoenmuang R, Khamsawads C, Methawanitphong N, Krutbut J, Makruen T, Rojanawiwat A. Insecticide susceptibility of genetically pyrethroid-resistant Aedes aegypti mosquitoes . Dis Control J [Internet]. 2021 Dec. 29 [cited 2024 Dec. 19];47(Suppl 2):1303-18. Available from: https://he01.tci-thaijo.org/index.php/DCJ/article/view/247959

Issue

Vol. 47 No. Suppl 2 (2021): October-December (Supplement 2)

Section

Original Article

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Articles published in the Disease Control Journal are considered as academic work, research or analysis of the personal opinion of the authors, not the opinion of the Thailand Department of Disease Control or editorial team. The authors must be responsible for their articles.