Insecticide susceptibility of genetically pyrethroid-resistant Aedes aegypti mosquitoes

Authors

  • Jakkrawarn Chompoosri National Institute of Health, Department of Medical Sciences
  • Ratana Tacharoenmuang National Institute of Health, Department of Medical Sciences
  • Chayada Khamsawads National Institute of Health, Department of Medical Sciences
  • Nittaya Methawanitphong National Institute of Health, Department of Medical Sciences
  • Jariya Krutbut National Institute of Health, Department of Medical Sciences
  • Thanyapak Makruen National Institute of Health, Department of Medical Sciences
  • Archawin Rojanawiwat National Institute of Health, Department of Medical Sciences

DOI:

https://doi.org/10.14456/dcj.2021.112

Keywords:

insecticide susceptibility, mutation, para gene, sodium channel protein, genetically pyrethroid-resistant Ae. aegypti mosquitoes

Abstract

Genetic resistance to pyrethroids in Aedes aegypti mosquitoes causes an ineffective use of pyrethroids for the control of Ae. aegypti-borne diseases. This reasearch aimed to study the insecticide susceptibility of genetically pyrethroid-resistant Ae. aegypti mosquitoes. Ae. aegypti mosquitoes from Nakhon Ratchasima province were exposed to 10 insecticides for susceptibility status by WHO susceptibility test. Polymerase chain reaction (PCR) and DNA sequencing were conducted for detecting mutations in target site for pyrethroids in domain II of sodium channel protein on para gene in pyrethroid-resistant Ae. aegypti mosquitoes. The results showed that Ae. aegypti mosquitoes from Nakhon Ratchasima province were completely susceptible to fenitrothion and malathion in organophosphates including propoxur and bendiocarb in carbamates with mean mortality rates at 24 h of 100±0%. However, the mosquitoes were resistant to all 6 pyrethroid insecticides with the mean mortality rates varied between 9.00±3.83% and 26.00±5.16%. The mean mortality rates of mosquitoes were significantly different among insecticides (p<0.05). Based on the analysis of 60 partial nucleotide sequences of para gene, 35 sequences were positive for two point mutations by amino acid substitutions of S989P and V1016G in sodium channel protein. The mutation frequency in the analyzed sequences of the pyrethroid-resistant mosquitoes was 0.58. It suggests that organophosphates and carbamates could be alternative insecticides to pyrethroids for the control of genetically pyrethroid-resistant Ae. aegypti mosquitoes. However, those insecticides should be used sparingly and integrated with the safe mosquito control measures.

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Published

2021-12-29

How to Cite

1.
Chompoosri จ, Tacharoenmuang ร, Khamsawads ช, Methawanitphong น, Krutbut จ, Makruen ธ, Rojanawiwat อ. Insecticide susceptibility of genetically pyrethroid-resistant Aedes aegypti mosquitoes . Dis Control J [Internet]. 2021 Dec. 29 [cited 2022 May 29];47(Suppl 2):1303-18. Available from: https://he01.tci-thaijo.org/index.php/DCJ/article/view/247959

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