Efficacy of lemongrass aerosol sprays against insecticide-resistant Aedes aegypti mosquitoes from dengue risk areas

Authors

  • Jakkrawarn Chompoosri National Institute of Health, Department of Medical Sciences
  • Chayada Khamsawads National Institute of Health, Department of Medical Sciences
  • Jariya Krutbut National Institute of Health, Department of Medical Sciences
  • Ratana Tacharoenmuang National Institute of Health, Department of Medical Sciences
  • Thanyapak Makruen National Institute of Health, Department of Medical Sciences
  • Archawin Rojanawiwat National Institute of Health, Department of Medical Sciences

DOI:

https://doi.org/10.14456/dcj.2022.9

Keywords:

lemongrass aerosol spray, insecticide-resistant Aedes aegypti, dengue risk areas

Abstract

An efficacy study of lemongrass aerosol spray against insecticide-resistant Aedes aegypti mosquitoes from dengue risk areas in Nakhon Pathom and Chanthaburi provinces was conducted through investigation. Lemongrass (Cymbopogon citratus) essential oil was extracted by hydrodistillation and its chemical composition was analyzed by Gas Chromatography-Mass Spectrometry (GC-MS). The oil was tested on susceptible Ae. aegypti mosquitoes to determine a discriminating concentration (2xLC99) and then tested against the insecticide-resistant mosquitoes by modification of the WHO insecticide susceptibility test. Lemongrass aerosol sprays were further developed and then studied for the efficacy against the insecticide-resistant mosquitoes in glass chamber and the fields. The results showed that the main constituents identified from the oil were geranial (28.77%) and neral (22.74%). The discriminating concentration of the oil was set at 0.814% that provided a 100% mortality rate for the insecticide-resistant mosquitoes. Lemongrass aerosol sprays containing the oil concentrations of 0.814%, 4.07%, 8.14% and 8.14% with flavoring agents gave the mortality rates of 0%, 30-40%, 100% and 100% for the insecticide-resistant mosquitoes from both provinces in glass chamber, respectively. The aerosol sprays containing the oil concentrations of 8.14% and 8.14% with flavoring agents provided the 100% mortality rate for Ae. aegypti mosquitoes in the fields of both provinces at 24 hours. In conclusion, the lemongrass aerosol sprays containing the oil concentrations of 8.14% and 8.14% with flavoring agents were effective against the insecticide-resistant Ae. aegypti mosquitoes from dengue risk areas of both provinces. It suggests that the lemongrass aerosol sprays could be alternative products to chemical aerosol sprays for mosquito control purpose.

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Published

2022-03-30

How to Cite

1.
Chompoosri J, Khamsawads C, Krutbut J, Tacharoenmuang R, Makruen T, Rojanawiwat A. Efficacy of lemongrass aerosol sprays against insecticide-resistant Aedes aegypti mosquitoes from dengue risk areas. Dis Control J [Internet]. 2022 Mar. 30 [cited 2024 Dec. 19];48(1):83-98. Available from: https://he01.tci-thaijo.org/index.php/DCJ/article/view/247257

Issue

Vol. 48 No. 1 (2022): January-March

Section

Original Article

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