Urinary chlorpyrifos metabolite concentrations and chlorpyrifos use behaviors among occupational sprayers in Suphan Buri Province


  • Sutatip Buranasatitnon คณะสาธารณสุขศาสตร์ มหาวิทยาลัยมหิดล
  • Pornpimol Kongtip
  • Noppanun Nankongnab
  • Mathuros Tipayamongkholgul
  • Attapon Cheepsattayakorn
  • Bunlert Bunerd




chlorpyrifos, urine, TCP, occupational sprayers, biomarkers


The urinary TCP (3,5,6-Trichloro-2-pyridinol) is widely accepted as the biomarker of chlorpyrifos exposure. This study is a cross sectional study. The objectives are to determine the urinary TCP concentrations in the morning void urine after spraying chlorphyrifos, and work behavior of spraying chlorpyrifos in the occupational sprayers. The subjects consisted of 82 occupational sprayers in Suphanburi Province, Thailand. The subjects were interviewed with questionnaire and urine samples were collected from occupational sprayers in the morning after spraying chlorpyrifos. Urine samples were analyzed with Gas Chromatograph/Mass Spectrometer (GC/ MS).The data were then analyzed using descriptive statistics and Mann Whitney U Test. The study found that TCP in all urine samples (100%) were between 15 to 136,716 µg/g creatinine. In addition, the study found the chlorpyrifos quantity used and behavior of standing under the wind while spraying chlorpyrifos increased the urinary TCP in the morning void urine (p<0.001 and p<0.05), respectively. In conclusion, the amount of chlorpyrifos receives to the body increases with the amount of chlorpyrifos sprayed, number of spraying event and behavior while spraying chlorpyrifos. Therefore, the substitution of chlorpyrifos should be in place, together with organizing the occupational health services in the community to follow up on health surveillance of impact on health of occupational sprayers and local farmers.


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How to Cite

Buranasatitnon ส, Kongtip พ, Nankongnab น, Tipayamongkholgul ม, Cheepsattayakorn อ, Bunerd บ. Urinary chlorpyrifos metabolite concentrations and chlorpyrifos use behaviors among occupational sprayers in Suphan Buri Province. Dis Control J [Internet]. 2020 Dec. 23 [cited 2022 Jun. 29];46(4):473-82. Available from: https://he01.tci-thaijo.org/index.php/DCJ/article/view/236872



Original Article