Validation of the Rapid Diagnostic Test for Dengue Fever/Dengue Hemorrhagic Fever which Detecting both Antigen and Antibody

Authors

  • วิชัย สติมัย Bureau of Vector Borne Disease, Department of Disease Control, Ministry of Public Health,
  • พงษ์วิทย์ บัวล้อมใบ Bureau of Vector Borne Disease, Department of Disease Control, Ministry of Public Health,
  • วิบูลย์ศักดิ์ วุฒิธนโชติ Chumpae Hospital, Kon Khan Province
  • ฑิตถากร รอดนาค Bureau of Vector Borne Disease
  • คนึงนิจ คงพ่วง Bureau of Vector Borne Disease
  • รุ่งระวี ทิพย์มนตรี Bureau of Vector Borne Disease

Keywords:

Dengue nonstructural protein 1 antigen, Rapid dengue diagnosis

Abstract

The objective of this study was to evaluate the rapid diagnostic test for Dengue and Dengue haemorrhagic fever (DF/DHF) which detecting RDT NS1 and RDT IgM/IgG (SD Bioline Dengue Duo). One hundred and seventy - three suspected DF/DHF cases from Chumpae Community Hospital, Khon Kean Province, Thailand during July - September 2009 were recruited into this study. The results showed this rapid diagnostic test detected 40 and 23 primary and secondary infective patients respectively. The sensitivity was 70.4 % and specificity was 89.9 % in acute phrase of illness while in convalescence phase, the sensitivity and specificity were 77.3 % and 86.3 % respectively. Totally, the test gave 71.4 % sensitivity and 88.9 % specificity to all patients. However, the RDT's sensitivity was deviated by the duration after onset of fever. The RDT NS1 showed the highest sensitivity in the third day ( 100 %) and the RDT IgM showed the highest sensitivity in the forth day ( 58 %). This rapid diagnostic test showed moderate sensitivity; nevertheless, it showed high specificity in both acute and convalescence phases of illness. In addition, its sensitivity showed the highest in the early phase of illness (the 3rd - 4th day after onset of illness).

References

1. สำนักโรคติดต่อนำโดยแมลง รายงานประจำปี 2550 กรมควบคุมโรค หน้า 87-94

2. อนันต์ นิสาลักษณ์ บทที่ 7 การวินิจฉัยทางห้องปฏิบัติการ ในไข้เลือดออกเด็งกี่ ไวรัสวิทยา พยาธิ กำเนิดจากกลไกภูมิคุ้มกัน การวินิจฉัย วัคซีน การป้องกัน และการควบคุม สมาคมไวรัสวิทยา (ประเทศไทย) ปี 2549 หน้า 173-174

3. Dussart P, Labeau B, Lagathu G, et al. Evaluation of an Enzyme Immunoassay for dectection of Dengue Virus NS1 Antigen in Human Serum. Clin Vaccine Immunol, 2006; 13 ( 11): 1185-1189.

4. Dussart P, Pertit L, Labeau B, et al. Evaluation of two new commercial tests for the diagnosis of acute dengue Virus infection using NS1 antigen detection in Human Serum. PLOS neglected Tropical Diseases 2008; 2 ( 8): 1-9.

5. Libraty DH, Endy TP, Houng HS, et al. Differing influences of virus burden and immune activation on disease severity in secondary dengue-3 virus infections. J Infect Dis 2002; 185: 1213-1221.

6. Shu PY, Chen LK, Chang SF, et al. Potential application of nonstructural protein NS1 serotype- specific immunoglobulin G Enzyme-linked immunosorbent assay in the seroepidemiologic study of dengue virus infection: correlation of results with those of the plaque reduction neutralization test . J Clin Microbiol 2002; 40: 1840-1844.

7. Alcon S, Talarmin A, Debruyne M, Falconar A, Deubel V, and Flamand M. Enzyme-linked immunosorbent assay specific to dengue virus type I nonstructural protein NS1 reveals circulation of the antigen in the blood during the acute phase of disease in patients experiencing primary or secondary infections. J Clin Microbiol 2002; 40: 376-381.

8. Shu PY, Chen LK, Chang SF, et al. Comparison of capture immunoglobulin M ( IgM) and IgG Enzyme-linked immunosorbent assay (ELISA) and nonstructural protein NS1 serotype-specific IgG ELISA for differentiation of primary and secondary virus infections. Clin Diagn Lab Immunol 2002; 10: 642-650.

9. Blacksell SD, Mammen MP Jr, Thongpaseuth S, et al. Evaluation of the Panbio dengue virus nonstructural I antigen detection and immunoglobulin M antibody Enzyme-linked immunosorbent assays for the diagnosis of acute infections in Laos. Diagn Microbiol Infect Dis 2008; 60: 43-49

10. Blacksell SD, Newton PN, Bell D, et al. The comparative accuracy of 8 commercial rapid immunochromatographic assays for the diagnosis of acute dengue virus infection. CID 2006; 41: 1127-1134.

11. Lam SK and Devine PL. Evaluation of capture ELISA and rapid immunochromatographic test for the determination of IgM and IgG antibodies produce during dengue infection. Clinical and Diagnostic Virology; 1998, 10, 75-81

12. Vajpayee M, Singh UB, Seth P and Broor S. Comparative evaluation of various commercial assays for diagnosis of dengue fever, Southeast Asian J Trop Med Public Health 2001;32(3): 472-475.

13. Mui Wang S and Devi Sekaran S. Early Diagnosis of Dengue Infection Using a Commercial Dengue Duo Rapid Test Kit for the Detection of NS1, IgM, and IgG. Am J Trop Med Hyg2010, 83(3), 2010, pp. 690-695

14. Osolio L, Ramires M, Bonelo A, Villar LA and Parra B. Comparison of the diagnostic accuracy of commercial NS1-based diagnostic tests for early dengue infection. Virol Journal 2010, 7: 361-382

Downloads

Published

2011-03-31

How to Cite

1.
สติมัย ว, บัวล้อมใบ พ, วุฒิธนโชติ ว, รอดนาค ฑ, คงพ่วง ค, ทิพย์มนตรี ร. Validation of the Rapid Diagnostic Test for Dengue Fever/Dengue Hemorrhagic Fever which Detecting both Antigen and Antibody. Dis Control J [Internet]. 2011 Mar. 31 [cited 2024 May 22];37(1):27-3. Available from: https://he01.tci-thaijo.org/index.php/DCJ/article/view/155604

Issue

Vol. 37 No. 1 (2011): January-March

Section

Original Article

License

Articles published in the Disease Control Journal are considered as academic work, research or analysis of the personal opinion of the authors, not the opinion of the Thailand Department of Disease Control or editorial team. The authors must be responsible for their articles.