Comparative study of simple smear, formalin ethyl acetate concentration and Parasep® faecal parasite concentration technique for identification of the parasite and opportunistic protozoa in stool specimen from HIV infected patients
DOI:
https://doi.org/10.14456/dcj.2013.6Keywords:
Protozoa, Opportunistic Protozoa, Stool, HIVAbstract
This Study aimed to compare the diagnostic yields for parasites and opportunistic parasites in stool samples using three methods: simple smear, formalin ethyl acetate concentration and Parasep® faecal parasite concentration. Two hundred stool samples of HIV-infected patients were collected for laboratory investigation at Bamrasnaradura Infectious Diseases Institute. The stool samples were observed under the light microscope by 3 methods using (1) direct wet smear, (2) iodine preparations and (3) modified Ziehl- Neelsen stain. The diagnostic yield for parasites and opportunistic parasites using Parasep® was higher than that of simple smear, formalin ethyl acetate concentration with the percentage of 10.5, 8.0 and 4.0, respec¬tively. The diagnostic yield for opportunistic parasites by modified Ziehl-Neelsen stain. The Parasep® and Simple smearwere higher than that of formalin ethyl acetate concentration with 10.0, 10.5 and 7.0, respec-tively. This study suggests that the Parasep® together with the standard simple smear can potentially enhance the laboratory reports for diagnosing protozoas and opportunistic parasites. The result indicates that Parasep® can be used to improve the diagnostic yields for protozoas and opportunistic parasites in stool samples.
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2. Wiwanitkit V. Intestinal parasitic infections in Thai HIV-infected patients with different im¬munity status. BMC Gastroenterology 2001; 1:3.
3. Babatunde SK, Salami AK, Fabiyi JP, Agbede oo, Desalu oo. Prevalence of intestinal para¬sitic infestation in HIV seropositive and serone¬gative patients in Ilorin, Nigeria. Ann Afr Med 2010;9:123-8.
4. Levine JA, Estevez EG. Method for concentra¬tion of parasites from small amounts of feces. J Clin Microbiol 1983;18:786-8.
5. Feleke M, Yeshambel B, Moges T, Yenew K, Andragachew M, Afework K, et al. Comparison of formol-acetone concentration method with that of the direct iodine preparation and formol-ether concentration methods for examination of stool parasites. Ethiop J Health Dev 2010;24:148- 51.
6. Kettelhut M, Moody A, Edwards H, Chiodini PL. Evaluation of Parasep® faecal parasite con¬centrator [Internet], [cited 2003 March 07]. Available at: http://www.diasys.com/PDF/ PARO 2 9 - HTD_Evaluation_Mkettelhut_ AMoody.pdf.
7. Moody A H, Sitara R, Mercedes ร. Evaluation of Parasep? SF, Ether and Ethyl Acetate Free Faecal Concentrators. [Internet], [cited 2011 April 05].Available at http://www.diasys.com/ PDF/ Evaluation_Document_ParasepSF.pdf.
8. Derrick B, Blundell N, Hart CA. The develop¬ment and performance of a simple, sensitive method for the detection of Cryptosporidium Oo¬cysts in faeces. Journal of Hygiene 1984;93: 317-23.
9. Kaplan JE, Benson c, Holmes KH, Brooks JT, Pau A, Masur H. Guidelines for prevention and treatment of opportunistic infections in HIV-in¬fected adults and adolescents: recommendations from CDC, the National Institutes of Health, and the HIV Medicine Association of the Infectious Diseases Society of America. MMWR 2009; 58:1-207.
10. Nelson M, Dockrell D, Edwards ร. British HIV Association (BHIVA) 'Guidelines for the treat¬ment of opportunistic infection in HIV-positive individuals 2010' (In consultation period). [Internet], [cited 2010 June 15]. Available at http://www.liv.ac.uk/hiv/2010_ BHIVA_OI- GuidelineConsultationVersion.pdf.
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