Epidemiology and genotypes of Mycobacterium tuberculosis at an outbreak area in Kanchanaburi province

Authors

  • อนุพงศ์ สุจริยากุล Office of Disease Prevention and Control Region 5, Ratchaburi Department of Disease Control
  • จณิศรา ฤดีอเนกสิน National Institute of Health, Department of Medical Sciences
  • ณัฏฐกัญจน์ ทิพย์เครือ Office of Disease Prevention and Control Region 5, Ratchaburi Department of Disease Control
  • เบญจวรรณ เพชรสุขศิริ National Institute of Health, Department of Medical Sciences

DOI:

https://doi.org/10.14456/dcj.2016.7

Keywords:

drug resistant tuberculosis, spoligotype, MIRU-VNTR

Abstract

Tuberculosis (TB) is one of the leading public health problems. Genotypic characterization of My¬cobacterium tuberculosis (MTB) is useful for epidemiological investigation and tracking of TB transmission. This study aimed to genetically identify and observe transmission of MTB by analyzing DNA fingerprints of tubercle bacilli isolated from pulmonary TB patients who were registered at an outbreak area of Thamaka district, Kanchanaburi province in 2013-2014. Sixty-eight DNA samples including twenty multidrug-resistant (MDR), and five mono-resistant MTB either to isoniazid or rifampicin were genotyped based on spoligo¬typing and subtyped using Mycobacterial interspersed repetitive unit-variable number of tandem repeat (MI¬RU-VNTR). The results revealed that 34 of 68 (50.00%) isolates belonged to Beijing genotype (SIT No. 1). Other twelve and seven new spoligotypes were identified. The major cumulative subtype of Beijing cluster was MDR-TB, and 14 of these had identical MIRU-VNTR genotype. This evidence linked to the strains of M. tuberculosis that have been found at an MDR-TB outbreak in Thamaka district, Kanchanaburi province since the year 2002. It suggests that this area still has MDR-TB transmission which cannot be eliminated over the time. MTB genotyping, proper measures of TB prevention and control should be contin¬ued.

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Published

2016-12-30

How to Cite

1.
สุจริยากุล อ, ฤดีอเนกสิน จ, ทิพย์เครือ ณ, เพชรสุขศิริ เ. Epidemiology and genotypes of Mycobacterium tuberculosis at an outbreak area in Kanchanaburi province. Dis Control J [Internet]. 2016 Dec. 30 [cited 2024 May 2];42(4):337-4. Available from: https://he01.tci-thaijo.org/index.php/DCJ/article/view/152393

Issue

Vol. 42 No. 4 (2016): October-December

Section

Original Article

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