TY - JOUR AU - Kanwichai, S. AU - Panasophonkul, S. AU - Bernard, J. K. AU - Suriyasathaporn, W. PY - 2021/02/01 Y2 - 2024/03/29 TI - Effect of daily environment and season in a tropical environment on the expression of heat shock protein, and steroidogenic and apoptotic genes in bovine cumulus-oocyte complex JF - The Thai Journal of Veterinary Medicine JA - TJVM VL - 51 IS - 2 SE - Original Articles DO - UR - https://he01.tci-thaijo.org/index.php/tjvm/article/view/247795 SP - 207-212 AB - <p><span class="fontstyle0">The objectives of this study were to determine the differences in the expression of heat shock protein, and<br>steroidogenic and apoptosis genes by cumulus-oocyte complex (COC) of beef cattle in a tropical environment as<br>affected by seasonal and day effect of temperature and relative humidity. Real-time reverse transcription-polymerase<br>chain reaction (RT-PCR) was used to measure gene expression in COC in different seasons including </span><span class="fontstyle0">cool (Jan to Feb),<br>summer (Mar to Apr) and rainy (May to Jul) seasons</span><span class="fontstyle0">. Ambient temperature and relative humidity recorded on the day<br>before oocyte collection were used to classify environment groups as: low temperature and low humidity (LT-LH),<br>high temperature and low humidity (HT-LH) and high temperature and high humidity (HT-HH). Expression of HSP70<br>was higher in the cool than rainy season, and for LT-LH than HT-HH (</span><span class="fontstyle2">p </span><span class="fontstyle0">&lt; 0.05). For the steroidogenic gene, STAR genes<br>were expressed less in HT-LH than either LT-LH or HT-HH (</span><span class="fontstyle2">p </span><span class="fontstyle0">&lt; 0.05), but no effect of season was observed. Expression<br>of MCL-1 was lower (</span><span class="fontstyle2">p </span><span class="fontstyle0">&lt; 0.05) for HT-LH than LT-LH or HT-HH and BAX was higher (</span><span class="fontstyle2">p </span><span class="fontstyle0">&lt; 0.05) for HT-LH than LTLH or HT-HH. Except for HSP70, expression of heat shock proteins and steroidogenic and apoptotic genes in COC was<br>not affected by season. However, expression of these genes in COC was altered when environmental conditions were<br>characterized by HT-LH. Higher humidity appears to mediate expression of these genes when climates are hot.</span> </p> ER -