TY - JOUR AU - Nur, Indriyani AU - Linianti, AU - Munaeni, Waode AU - Abidin, La Ode Baytul AU - Maulidiyah, PY - 2020/11/09 Y2 - 2024/03/29 TI - Assessment of antibacterial and immunostimulating activity of black cumin (Nigella sativa) extract against vibriosis in white shrimp (Litopenaeus vannamei) JF - The Thai Journal of Veterinary Medicine JA - TJVM VL - 50 IS - 4 SE - Original Articles DO - UR - https://he01.tci-thaijo.org/index.php/tjvm/article/view/246367 SP - 549-557 AB - <p><span class="fontstyle0">It is assumed that the active components of Black cumin seed (</span><span class="fontstyle2">Nigella sativa</span><span class="fontstyle0">) contain beneficial effects on shrimp<br>health as in humans. Therefore, this study aims to explore the antibacterial and immunostimulant potential of black<br>cumin extract for disease control across </span><span class="fontstyle2">V. harveyi</span><span class="fontstyle0">. Treatments applied include the administration of ethanol extract<br>mixed into shrimp feed at varying dosages (2500, 5000, 7500 ppm), hereinafter referred to as treatments A, B, C,<br>respectively. All shrimps were exposed to pathogen </span><span class="fontstyle2">V. harveyi </span><span class="fontstyle0">at a density of 10</span><span class="fontstyle0">6 </span><span class="fontstyle0">CFU ml</span><span class="fontstyle0">-1 </span><span class="fontstyle0">after 14 days of testing<br>duration excluding the negative control group. The immune responses of the shrimps were recognized as Average<br>Total Haemocyte Count (THC) and Differential Haemocyte Count (DHC). In addition, the vibrio challenge test was<br>applied to assess the resistance of shrimp and the population of bacteria and Relative Percent Survival (RPS) were<br>consequently determined. The results indicated that the highest THC, the best proportion in both granula cells and<br>hyalin cells, and the highest RPS (68%) was found in the treatment of 7500 ppm compared to other treatments at all<br>sampling times. Also, it was able to suppres the proliferation of </span><span class="fontstyle2">V. harveyi </span><span class="fontstyle0">bacteria more prominently in the shrimp gut.<br>Extract addition in the diet can control vibriosis especially at a dose of 7500 ppm, indicated by a decrease in the bacterial<br>population and a better immune response. Therefore, extract of black cumin seeds has antibacterial as well as<br>immunostimulant activity.</span> </p> ER -