TY - JOUR AU - Tahir, Tehreem AU - Ashfaq, Muhammad AU - Shahzad, Mirza Imran AU - Tabassum, Rukhsana PY - 2020/09/21 Y2 - 2024/03/28 TI - Antiviral evaluation of bioactive azo derivatives to treat endemic poultry viruses JF - The Thai Journal of Veterinary Medicine JA - TJVM VL - 50 IS - 3 SE - Short Communications DO - UR - https://he01.tci-thaijo.org/index.php/tjvm/article/view/245854 SP - 435-443 AB - <p><span class="fontstyle0">The present study focused on the synthesis, </span><span class="fontstyle2">In Ovo </span><span class="fontstyle0">antiviral evaluation and </span><span class="fontstyle2">In silico </span><span class="fontstyle0">study of most active antiviral<br>compounds of the azo series. The synthesis of title compounds was done by the coupling reaction of diazonium salt<br>solutions with active methylene (1,3-dioxolane and benzimidazole), to yield [(</span><span class="fontstyle2">E</span><span class="fontstyle0">)-1-(1,3-dioxolan-2-yl)-2-phenyldiazene]<br>(</span><span class="fontstyle3">A1</span><span class="fontstyle0">), [(</span><span class="fontstyle2">E</span><span class="fontstyle0">)-1-(1,3-dioxolan-2-yl)-2-(4-methyl-phenyl)diazene] (</span><span class="fontstyle3">A2</span><span class="fontstyle0">), 2-[(</span><span class="fontstyle2">E</span><span class="fontstyle0">)-phenyldiazenyl]-1</span><span class="fontstyle2">H</span><span class="fontstyle0">-benzimidazole] (</span><span class="fontstyle3">A3</span><span class="fontstyle0">) ,<br>[(</span><span class="fontstyle2">E</span><span class="fontstyle0">)-1-(1,3-dioxolan-2-yl)-2-(4-ethylphenyl)diazene] (</span><span class="fontstyle3">A4</span><span class="fontstyle0">) and [(</span><span class="fontstyle2">E</span><span class="fontstyle0">)-1-(1,3-dioxolan-2-yl)-2-(2-methylphenyl)diazene]<br>(</span><span class="fontstyle3">A5</span><span class="fontstyle0">). The structures of newly synthesized molecules were elucidated by spectroscopic techniques (EI-MS and FT-IR).<br></span><span class="fontstyle2">In Ovo </span><span class="fontstyle0">screening of compounds against avian influenza virus (AIV) H9N2 strain and Newcastle Disease virus (NDV)<br>Lasota strain was done. The evaluation data suggested that azo compound (</span><span class="fontstyle3">A5</span><span class="fontstyle0">) exhibited the highest anti-AIV and<br>anti-NDV activity (100% inhibition at 0.1 mg/100 µL) compared to the other azo compounds which showed less activity<br>at given concentrations. Docking study further suggested that azo compound (</span><span class="fontstyle3">A5</span><span class="fontstyle0">) binds with the active site residues<br>of viral proteins with good binding affinity (-6.9 and -8.0 kcal/mol) compared to the standard oseltamivir due to the<br>substitution of -CH</span><span class="fontstyle0">3 </span><span class="fontstyle0">group at ortho position on the phenyl ring. Hence, based on this examination, it was concluded<br>that azo compound (</span><span class="fontstyle3">A5</span><span class="fontstyle0">) may act as a platform for designing more active antiviral agents.</span> </p> ER -