TY - JOUR AU - Hassanien, Rabab Taha AU - El-Nahas, Ehab Mostafa AU - Mahmoud, Nahed Ahmed AU - El-Bagoury, Gabr Fikry PY - 2020/09/21 Y2 - 2024/03/29 TI - Molecular characterization of equine herpesvirus-1 and asinine herpesvirus -5 isolated from aborted fetuses of arabian horses JF - The Thai Journal of Veterinary Medicine JA - TJVM VL - 50 IS - 3 SE - Original Articles DO - UR - https://he01.tci-thaijo.org/index.php/tjvm/article/view/245850 SP - 397-403 AB - <p><span class="fontstyle0">Infections with equine herpesviruses (EHVs) are widespread in equine populations worldwide. Whilst EHV-1<br>produces well-documented reproductive losses in equids, the role of asinine herpesviruses-5 (AHV-5) to induce<br>abortion is still enigmatic. In the present study, EHV-1 and AHV-5 were detected in aborted fetuses of Arabian horses<br>in Egypt by consensus nested PCR of DNA polymerase gene, with a trial for viral isolation on Rabbit Kidney 13 cell<br>line (RK-13) and identification by indirect immunofluorescence. The partial analysis of DNA polymerase gene sequence<br>revealed that the Egyptian strains EHV-1 and AHV-5 were related to Australian origin EHV-1 838/89 (Accession. no,<br>KF434370) and Austrian strain AHV-5 isolate 1323 (Accession. no, KC825357) by 98.3% and 98.6 % respectively. In<br>conclusion, this was the first detection and isolation of AHV-5 in Egypt, and to our knowledge, this represents the first<br>report of AHV-1 detection from an aborted fetus worldwide.</span> </p> ER -