TY - JOUR AU - Sajjacharoenpong, Kriengyot AU - Chuesiri, Pawana PY - 2020/06/30 Y2 - 2024/03/30 TI - Effectiveness of embalming solutions with partial replacement of formaldehyde and phenol with ethanol and sodium chloride on mice over 18 months JF - The Thai Journal of Veterinary Medicine JA - TJVM VL - 50 IS - 2 SE - Original Articles DO - UR - https://he01.tci-thaijo.org/index.php/tjvm/article/view/244189 SP - 233-238 AB - <p><span class="fontstyle0">Animal preservation for anatomy teaching has been required to replace formaldehyde and phenol which represent<br>a health hazard to humans. A comparative study of embalming solutions with partial replacement of formaldehyde<br>and phenol with sodium chloride and ethanol was evaluated in mouse cadavers over an 18-month period. Twelve adult<br>mouse carcasses were divided into six groups of two mice each. After opening the mouse’s skin, the internal organs<br>were removed and preserved with the remaining cadavers by freezing (Group I, control group) or in the respective<br>embalming solution (Groups II to VI) and examined after 3, 6, 12 and 18 months. For the embalming solution, the<br>formaldehyde concentration was reduced from 18% (Group II) to 0.5% (Groups III and IV), 0.1% (Group V), and 0.05%<br>(Group VI), whereas the phenol and glycerol concentration was reduced from 2.5% and 5% (Group II) to 0.8% and 2%,<br>(Groups III-VI), respectively. In replacement, 15% table salt/sea salt and 16% ethanol were included in Groups III and<br>IV. The carcasses and internal organs were blind evaluated by 10 students. The evaluations were compared and<br>analyzed between Groups and against Group 1 using ANOVA. Results show that embalmed mice in Groups III and IV<br>retained a good color and flexibility, without decomposition or fungal growth throughout study. In contrast, mice in<br>Groups V and VI were unsuitable at 3 months. We concluded that a suitable embalming fluid for small animal<br>preservation was 0.5% formaldehyde, 0.8% phenol, 2% glycerol, 16% ethanol and 15% sodium chloride.</span> </p> ER -