TY - JOUR AU - Ting, Chiu-Huang AU - Lin, Chia-Ying AU - Chung, Chia-Wen AU - Shen, Perng-Chih AU - Chang, Ching-Dong AU - Wu, Hung-Yi PY - 2020/06/30 Y2 - 2024/03/29 TI - Prevalence of Salmonella spp. and Escherichia coli in dogs with diarrhoea in Western Taiwan JF - The Thai Journal of Veterinary Medicine JA - TJVM VL - 50 IS - 2 SE - Original Articles DO - UR - https://he01.tci-thaijo.org/index.php/tjvm/article/view/244083 SP - 227-232 AB - <p><span class="fontstyle0">Salmonella </span><span class="fontstyle2">spp. can be widely spread by air or contaminated food. There are six common pathogenic </span><span class="fontstyle0">Escherichia coli<br></span><span class="fontstyle2">which account for 4–8% of all </span><span class="fontstyle0">E. coli </span><span class="fontstyle2">pathogens. Amongst them, </span><span class="fontstyle0">E. coli </span><span class="fontstyle2">O157 is the most commonly known. In this study,<br>we investigated the prevalence of diarrhoea pathogens, referred to as </span><span class="fontstyle0">Salmonella </span><span class="fontstyle2">spp. and </span><span class="fontstyle0">Escherichia coli </span><span class="fontstyle2">(</span><span class="fontstyle0">E. coli</span><span class="fontstyle2">), in pet<br>dogs in western Taiwan using the conventional polymerase chain reaction (PCR). The related analysis between the<br>prevalence rates and the epidemiological data of pet dogs was correlated with age, season, area and breed. </span><span class="fontstyle0">Faecal<br></span><span class="fontstyle2">samples were collected from </span><span class="fontstyle0">240 </span><span class="fontstyle2">dogs which had symptoms of diarrhoea and had been examined by </span><span class="fontstyle0">veterinary </span><span class="fontstyle2">hospitals<br>from January 2015 to January 2017. The </span><span class="fontstyle0">PCR sensitivity </span><span class="fontstyle2">of the total deoxyribonucleic acids (DNAs) extracted from 0.1g<br>faecal samples ranging from 10 fg to 100 ng was examined. The prevalence of </span><span class="fontstyle0">Salmonella </span><span class="fontstyle2">spp. and </span><span class="fontstyle0">E. coli </span><span class="fontstyle2">infections was<br>19.2% (46/240) and 3.3% (8/240), respectively. Results showed that </span><span class="fontstyle0">Salmonella </span><span class="fontstyle2">spp. was at its most prevalent during<br>spring, whilst </span><span class="fontstyle0">E. coli </span><span class="fontstyle2">was most prevalent in summer, and the highest proportions might occur in puppies in suburban<br>dogs. Since both diseases are zoonotic, the more humans are exposed to it, the higher its occurrence will be. This study<br>has provided clinical veterinarians with the advanced ability to diagnose both diseases and with crucial information<br>for the prevalence of </span><span class="fontstyle0">Salmonella </span><span class="fontstyle2">spp. and </span><span class="fontstyle0">E. coli </span><span class="fontstyle2">diseases in dogs with diarrhoea in Taiwan.</span> </p> ER -