TY - JOUR AU - Alzuheir, Ibrahim AU - Jalboush, Nasr AU - Fayyad, Adnan AU - Manassra, Mohammad PY - 2020/06/30 Y2 - 2024/03/29 TI - Sequence and Phylogenetic analysis of the VP1 segment of chicken infectious anemia virus (CIAV) during an outbreak in Palestine JF - The Thai Journal of Veterinary Medicine JA - TJVM VL - 50 IS - 2 SE - Original Articles DO - UR - https://he01.tci-thaijo.org/index.php/tjvm/article/view/244081 SP - 219-225 AB - <p><span class="fontstyle0">In this study, chicken infectious anemia virus (CIAV) was detected in two unvaccinated commercial broiler flocks,<br>showing clinical signs relevant to Chicken infectious anemia (CIA) disease. The clinical signs included subcutaneous<br>hemorrhage, depression and death. Necropsy findings were pale liver, severe atrophy of bursa of the Fabricius and<br>thymus and discoloration of the bone marrow. DNA was extracted from the livers of the infected birds and the VP1<br>gene was detected by polymerase chain reaction. The virus strains were detected and genetically characterized in<br>almost the whole VP 1 gene (1338 base pairs). The nucleic acid analysis showed that the two Palestinian strains had<br>99.9% similarity and 98.7% similarity to a strain from chickens in Bangladesh (Accession no. AF395114) and 98.5% to a<br>strain from a human fecal sample (Accession no. JQ690762) from China in the same cluster. The lowest similarity<br>(94.7%) was with a strain isolated from chickens in China (Accession no. KU645525). Alignment of the deduced VP1<br>amino acids showed that the two Palestinian strains shared 100% homology (with one silent mutation encoded Leucine<br>at residue no 97(</span><span class="fontstyle0">97</span><span class="fontstyle0">L)), classified as high pathogenicity based on glutamine at residue in position 394 (</span><span class="fontstyle0">394</span><span class="fontstyle0">Q). The highest<br>similarity was with the Bangladesh 99.8% (AAM20899), Brazil 99.5% (APQ44719) and China (AAZ40209) 99.5% strains,<br>while the lowest was with the China strain (AFR46599) 96.6%. The study provided valuable information on the<br>molecular characterization of CIAV strains in Palestine for the first time.</span> </p> ER -