TY - JOUR AU - Sananmuang, Thanida AU - Singto, Thitirat AU - Intarak, Kanyavee AU - Sanansin, Pimnipa AU - Budsasom, Muttana AU - Kongthip, Arrita AU - Wannaratana, Suwarak PY - 2020/06/30 Y2 - 2024/03/29 TI - Optimizing system for sperm quality evaluation using crystalloid diluent for pigeons (Columba livia domestica) JF - The Thai Journal of Veterinary Medicine JA - TJVM VL - 50 IS - 2 SE - Original Articles DO - UR - https://he01.tci-thaijo.org/index.php/tjvm/article/view/244075 SP - 203-209 AB - <p><span class="fontstyle0">Semen quality is a crucial factor for predicting fertility and hatchability in several avian species including pigeons.<br>To evaluate pigeon semen quality, prior dilution of semen was required due to its small volume with highly<br>concentrated spermatozoa. Conventional crystalloid solutions—Normal saline (NSS) and Ringer’s solution (LRS) are<br>common diluents for such a task; however, the unoptimized dilution rate of such diluents was a major cause<br>contributing to inaccurate semen quality evaluation. Due to the lack of such knowledge in pigeons this study aimed to<br>optimize the dilution rate of Normal saline (NSS) and Ringer’s solution (LRS) for pigeon semen quality evaluation. The<br>semen of a total of 20 male racing pigeons was collected and diluted in NSS or LRS at 12.5-, 25-, 50-, 100- and 200-fold<br>dilutions. Immediate percentages of sperm motility, progressive motility and viability were determined for each fold<br>dilution. The 12.5- and 25-fold-diluted semen were further evaluated at 0, 0.5, 1 and 3 h of storage. The results showed<br>that NSS and LRS rendered indifferent effects on immediate semen quality. However fold dilutions higher than 25<br>resulted in a great decline in semen quality both in NSS and LRS. Storage of 12.5-fold and 25-fold diluted semen resulted<br>in a marked decrease of semen quality as soon as 0.5 h; however, LRS was better than NSS in terms of maintaining<br>semen quality. This study thus supports immediate quality evaluation of pigeon semen—by which 12.5-25 folddilutions were suggested.</span> </p> ER -