TY - JOUR AU - Aiemsaard, Jareerat AU - Kamollerd, Chuchat AU - Suwannathada, Pimchanok AU - Thongkham, Eakachai PY - 2020/06/30 Y2 - 2024/03/28 TI - Antimicrobial activity of formulated clove essential oil spray against biofilm-forming Malassezia pachydermatis and Staphylococcus pseudintermedius clinical isolates JF - The Thai Journal of Veterinary Medicine JA - TJVM VL - 50 IS - 2 SE - Original Articles DO - UR - https://he01.tci-thaijo.org/index.php/tjvm/article/view/244071 SP - 185-191 AB - <p><span class="fontstyle0">Yeasts and bacteria that cause infectious skin diseases in animals are becoming increasingly resistant to<br>antimicrobial drugs, especially biofilm-forming microbial strains. Therefore, there is extensive interest in new effective<br>antimicrobial substances. Clove essential oil has broad-spectrum antimicrobial activity and can be developed into<br>various dosage forms. This study investigated the antimicrobial effects of a lipid-based spray containing 5% w/w clove<br>essential oil against biofilm-forming </span><span class="fontstyle2">Malassezia pachydermatis </span><span class="fontstyle0">and </span><span class="fontstyle2">Staphylococcus pseudintermedius </span><span class="fontstyle0">clinical isolates by<br>time-kill test. In addition, the physical stability and antimicrobial activity of the spray was assessed under accelerated<br>storage conditions. The time kill tests revealed that the prepared formulation decreased the number of viable yeast and<br>bacteria by more than 99.9999% (6-log reduction) within 15 min</span><span class="fontstyle0">s</span><span class="fontstyle0">. After storage at 40</span><span class="fontstyle0">0</span><span class="fontstyle0">C and 75% RH for 4 months, the<br>spray showed a slight increase in acidity and a slight darkening in colour. The antimicrobial and bactericidal activity<br>was unaffected but the anti-yeast activity diminished slightly in the </span><span class="fontstyle3">3 </span><span class="fontstyle0">rd </span><span class="fontstyle0">and </span><span class="fontstyle3">4 </span><span class="fontstyle0">th </span><span class="fontstyle0">months under accelerated storage<br>conditions. These results demonstrate the efficacy of clove essential oil lipid-based spray against biofilm-forming </span><span class="fontstyle2">M</span><span class="fontstyle4">.<br></span><span class="fontstyle2">pachydermatis </span><span class="fontstyle0">and </span><span class="fontstyle2">S. pseudintermedius</span><span class="fontstyle0">.</span> </p> ER -