TY - JOUR AU - Zeb, Saqib AU - Ali, Nawab AU - Niaz, Sadaf AU - Rasheed, Arsalan AU - Khattak, Irfan AU - Khan, Naimat Ullah AU - Wang, Yachun AU - Usman, Tahir PY - 2020/05/12 Y2 - 2024/03/29 TI - Association of SNPs in the coding regions of CD4 gene with mastitis susceptibility and production traits in dairy cattle JF - The Thai Journal of Veterinary Medicine JA - TJVM VL - 50 IS - 1 SE - Original Articles DO - UR - https://he01.tci-thaijo.org/index.php/tjvm/article/view/243259 SP - 75-80 AB - <p><span class="fontstyle0">CD4 </span><span class="fontstyle2">gene is reported to have significant role in various inflammatory conditions including mastitis. Therefore, the<br>current study was designed to screen out single nucleotide polymorphisms (SNPs) in the bovine </span><span class="fontstyle0">CD4 </span><span class="fontstyle2">gene and to<br>evaluate its effects on mastitis indicator and production traits. Total 201 milk and blood samples were collected from<br>dairy cattle maintained at government dairy farms of Khyber Pakhtunkhwa, Pakistan. Milk samples were used for DHI<br>test (milk composition and somatic cell count) and the blood for DNA extraction. SNPs were initially detected in the<br>pool DNA samples followed by screening in the whole cohort. The association of SNPs with the studied phenotypic<br>traits was analysed using the GLM procedure of SAS 9.4. Total four SNPs including three in exon 2 (SNP 1 A&gt;C<br>rs110955838, SNP 2 T&gt;G rs134722546, SNP 3 T&gt;C rs135440143) and one in exon four (SNP4 G&gt;A, rs134369392) were<br>revealed. The results showed that all the SNPs were significantly associated with frequency of clinical mastitis incidence<br>(</span><span class="fontstyle0">P</span><span class="fontstyle2">&lt;0.05). Only SNP4 (G&gt;A) was found to have significant association with annual milk yield (</span><span class="fontstyle0">P</span><span class="fontstyle2">&lt;0.05). Furthermore,<br>breed and herd showed significant association with mastitis indicator and production traits (</span><span class="fontstyle0">P</span><span class="fontstyle2">&lt;0.005). The parity wise<br>milk production results revealed highest annual milk yield in cattle with first parity (3649.2 L) followed by 2</span><span class="fontstyle2">nd </span><span class="fontstyle2">(3412.21<br>L), 3</span><span class="fontstyle2">rd </span><span class="fontstyle2">and above parities (3112.32 L). The present study suggests that </span><span class="fontstyle0">CD4 gene </span><span class="fontstyle2">should be considered as a candidate<br>gene and the identified SNPs could be worthy molecular markers for selection of dairy cattle with genetic resistance to<br>mastitis development.</span> </p> ER -